The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells

• 資料種別: 博士論文
• タイトル: The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells
• 著者・編者: ⽚⼭, 暢仁
• 著者標目: ⽚⼭, 暢仁
• 並列タイトル等: ヒト間葉系幹細胞に対するエナメルマトリックス由来合成ペプチドの影響
• 授与機関名: 大阪歯科大学
• 授与年月日: 2015-03-06
• 報告番号: 甲第744号
• 学位: 博士（歯学）
• 本文の言語コード: eng
• 件名標目: Alkaline Phosphatase; Cell Differentiation; Cell Proliferation; Cells, Cultured; Collagen Type I; Dental Enamel; Flavonoids; Humans; Mesenchymal Stromal Cells; Oligopeptides; Osteocalcin
• 対象利用者: 一般
• 一般注記: Enamel matrix derivative (EMD) is widely used in periodontal tissue regeneration therapy. However, because the bioactivity of EMD varies from batch to batch, and the use of a synthetic peptide could avoid use from an animal source, a completely synthetic peptide (SP) containing the active component of EMD would be useful. In this study an oligopeptide synthesized derived from EMD was evaluated for whether it contributes to periodontal tissue regeneration. We investigated the effects of the SP on cell proliferation and osteoblast differentiation of human mesenchymal stem cells (MSCs), which are involved in tissue regeneration. MSCs were treated with SP (0 to 1000 ng/mL), to determine the optimal concentration. We examined the effects of SP on cell proliferation and osteoblastic differentiation indicators such as alkaline phosphatase activity, the production of procollagen type 1 C-peptide and osteocalcin, and on mineralization. Additionally, we investigated the role of extracellular signal-related kinases (ERK) in cell proliferation and osteoblastic differentiation induced by SP. Our results suggest that SP promotes these processes in human MSCs, and that ERK inhibitors suppress these effects. In conclusion, SP promotes cell proliferation and osteoblastic differentiation of human MSCs, probably through the ERK pathway.; 2014年度
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: CC BY-NC-ND
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 大阪歯科大学 : 大阪歯科大学学術リポジトリ

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https://osaka-dent.repo.nii.ac.jp/?action=repository_action_common_download&item_id=15&item_no=1&attribute_id=20&file_no=1 fulltext

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