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博士論文

The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells

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The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells

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博士論文
Author
⽚⼭, 暢仁
Publisher
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Publication date
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Material Format
Digital
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Name of awarding university/degree
大阪歯科大学,博士（歯学）
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出版タイプ： VoREnamel matrix derivative (EMD) is widely used in periodontal tissue regeneration therapy. However, because the bioactivity of EMD varies fro...

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  • Osaka Dental University Academic Repository

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Digital

Material Type
博士論文
Title
The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells
Author/Editor
⽚⼭, 暢仁
Alternative Title
ヒト間葉系幹細胞に対するエナメルマトリックス由来合成ペプチドの影響
Degree grantor/type
大阪歯科大学
Dissertation Number
甲第744号
Degree Type
博士（歯学）
Subject Heading
Alkaline Phosphatase
Cell Differentiation
Cell Proliferation
Cells, Cultured
Collagen Type I
Dental Enamel
Flavonoids
Humans
Mesenchymal Stromal Cells
Oligopeptides
Osteocalcin
Target Audience
一般
Note (General)
出版タイプ： VoR
Enamel matrix derivative (EMD) is widely used in periodontal tissue regeneration therapy. However, because the bioactivity of EMD varies from batch to batch, and the use of a synthetic peptide could avoid use from an animal source, a completely synthetic peptide (SP) containing the active component of EMD would be useful. In this study an oligopeptide synthesized derived from EMD was evaluated for whether it contributes to periodontal tissue regeneration. We investigated the effects of the SP on cell proliferation and osteoblast differentiation of human mesenchymal stem cells (MSCs), which are involved in tissue regeneration. MSCs were treated with SP (0 to 1000 ng/mL), to determine the optimal concentration. We examined the effects of SP on cell proliferation and osteoblastic differentiation indicators such as alkaline phosphatase activity, the production of procollagen type 1 C-peptide and osteocalcin, and on mineralization. Additionally, we investigated the role of extracellular signal-related kinases (ERK) in cell proliferation and osteoblastic differentiation induced by SP. Our results suggest that SP promotes these processes in human MSCs, and that ERK inhibitors suppress these effects. In conclusion, SP promotes cell proliferation and osteoblastic differentiation of human MSCs, probably through the ERK pathway.
2014年度