The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells

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The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells

Material type: 博士論文

Author: ⽚⼭, 暢仁

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Name of awarding university/degree: 大阪歯科大学,博士（歯学）

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Note (General)：: Enamel matrix derivative (EMD) is widely used in periodontal tissue regeneration therapy. However, because the bioactivity of EMD varies from batch to...

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Material Type: 博士論文
Title: The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells
Author/Editor: ⽚⼭, 暢仁
Author Heading: ⽚⼭, 暢仁
Alternative Title: ヒト間葉系幹細胞に対するエナメルマトリックス由来合成ペプチドの影響
Degree grantor/type: 大阪歯科大学
Date Granted: 2015-03-06
Dissertation Number: 甲第744号
Degree Type: 博士（歯学）
Text Language Code: eng
Subject Heading: Alkaline Phosphatase
Cell Differentiation
Cell Proliferation
Cells, Cultured
Collagen Type I
Dental Enamel
Flavonoids
Humans
Mesenchymal Stromal Cells
Oligopeptides
Osteocalcin
Target Audience: 一般
Note (General): Enamel matrix derivative (EMD) is widely used in periodontal tissue regeneration therapy. However, because the bioactivity of EMD varies from batch to batch, and the use of a synthetic peptide could avoid use from an animal source, a completely synthetic peptide (SP) containing the active component of EMD would be useful. In this study an oligopeptide synthesized derived from EMD was evaluated for whether it contributes to periodontal tissue regeneration. We investigated the effects of the SP on cell proliferation and osteoblast differentiation of human mesenchymal stem cells (MSCs), which are involved in tissue regeneration. MSCs were treated with SP (0 to 1000 ng/mL), to determine the optimal concentration. We examined the effects of SP on cell proliferation and osteoblastic differentiation indicators such as alkaline phosphatase activity, the production of procollagen type 1 C-peptide and osteocalcin, and on mineralization. Additionally, we investigated the role of extracellular signal-related kinases (ERK) in cell proliferation and osteoblastic differentiation induced by SP. Our results suggest that SP promotes these processes in human MSCs, and that ERK inhibitors suppress these effects. In conclusion, SP promotes cell proliferation and osteoblastic differentiation of human MSCs, probably through the ERK pathway.
2014年度
Source: fulltext
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Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
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Original Data Provider (Database): 大阪歯科大学 : 大阪歯科大学学術リポジトリ