並列タイトル等破骨細胞形成とp38 MAP kinase経路に対するGallotanninの影響
一般注記We examined the effect of gallotannin on osteoclast differentiation in vitro. We found that gallotannin suppressed osteoclast differentiation in a bone marrow culture system stimulated with 1, 25 (OH)2 D3, as well as in a M-CSF/RANKL-treated BMM culture system. Also, we found that gallotannin treatment suppressed the expression of NFATc1 in RANKL-treated BMMs. To further elucidate the mechanisms underlying the suppressive effect of gallotannin on osteoclast differentiation, we examined the effects of gallotannin on RANKL- and M-CSF-mediated intracellular signaling. Although gallotannin did not affect the activation kinetics of ERK1/2 and Akt, it blunted p38 MAPK activation in RANKL-treated BMMs. We also observed the suppressive effects of gallotannin on M-CSF-induced p38 MAPK activation in BMMs that had been programmed for osteoclast differentiation by pretreatment with RANKL and low-dose M-CSF for 24 h. In other words, gallotannin specifically suppressed RANKL- and M-CSF-induced activation of the p-38 MAPK pathway.
2016年度
連携機関・データベース国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）