Bone Regeneration by Dedifferentiated Fat Cells Using Composite Sponge of Alfa-Tricalcium Phosphate and Gelatin in a Rat Calvarial Defect Model

• 資料種別: 博士論文
• タイトル: Bone Regeneration by Dedifferentiated Fat Cells Using Composite Sponge of Alfa-Tricalcium Phosphate and Gelatin in a Rat Calvarial Defect Model
• 著者・編者: 妻野, 誠仁
• 著者標目: 妻野, 誠仁
• 並列タイトル等: ラット頭蓋冠欠損モデルにおける-リン酸三カルシウムとゼラチンの複合スポンジを用いた脱分化脂肪細胞による骨再生
• 授与機関名: 大阪歯科大学
• 授与年月日: 2022-03-04
• 報告番号: 甲第938号
• 学位: 博士（歯学）
• 本文の言語コード: eng
• 件名標目: bone regeneration; alfa-tricalcium phosphate; animal study; dedifferentiated fat cells; rat calvar-ial defect
• 対象利用者: 一般
• 一般注記: Mechanical and resorbable scaffolds are in high demand for stem cell-based regenerative medi-cine, to treat refractory bone defects in craniofacial abnormalities and injuries. Multipotent pro-genitor cells, such as dedifferentiated fat (DFAT) cells, are prospective sources for regenerative therapies. Herein, we aimed to demonstrate that a composite gelatin sponge (α-TCP/GS) of al-fa-tricalcium phosphate (α-TCP) mixed with gelatin scaffolds (GS), with/without DFATs, induced bone regeneration in a rat calvarial defect model in vivo. α-TCP/GS was prepared by mixing α-TCP and 2% GS using vacuum-heated methods. α-TCP/GS samples with/without DFATs were transplanted into the model. After 4 weeks of implantation, the samples were subjected to mi-cro-computed tomography (μ-CT) and histological analysis. α-TCP/GS possessed adequate me-chanical strength; α-TCP did not convert to hydroxyapatite upon contact with water, as deter-mined by X-ray diffraction. Moreover, stable α-TCP/GS was formed by electrostatic interactions, and verified based on the infrared peak shifts. μ-CT analyses showed that bone formation was higher in the α-TCP/GS+ DFAT group than in the α-TCP/GS group. Therefore, the implantation of α-TCP/GS comprising DFAT cells enhanced bone regeneration and vascularization, demon-strating the potential for healing critical-sized bone defects.; 2021年度
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: CC BY-NC-ND
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 大阪歯科大学 : 大阪歯科大学学術リポジトリ

目次

https://osaka-dent.repo.nii.ac.jp/?action=repository_action_common_download&item_id=336&item_no=1&attribute_id=20&file_no=2 fulltext

https://osaka-dent.repo.nii.ac.jp/?action=repository_action_common_download&item_id=336&item_no=1&attribute_id=20&file_no=1 fulltext