Bone Regeneration by Dedifferentiated Fat Cells Using Composite Sponge of Alfa-Tricalcium Phosphate and Gelatin in a Rat Calvarial Defect Model

• Material Type: 博士論文
• Title: Bone Regeneration by Dedifferentiated Fat Cells Using Composite Sponge of Alfa-Tricalcium Phosphate and Gelatin in a Rat Calvarial Defect Model
• Author/Editor: 妻野, 誠仁
• Author Heading: 妻野, 誠仁
• Alternative Title: ラット頭蓋冠欠損モデルにおける-リン酸三カルシウムとゼラチンの複合スポンジを用いた脱分化脂肪細胞による骨再生
• Degree grantor/type: 大阪歯科大学
• Date Granted: 2022-03-04
• Dissertation Number: 甲第938号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: bone regeneration; alfa-tricalcium phosphate; animal study; dedifferentiated fat cells; rat calvar-ial defect
• Target Audience: 一般
• Note (General): Mechanical and resorbable scaffolds are in high demand for stem cell-based regenerative medi-cine, to treat refractory bone defects in craniofacial abnormalities and injuries. Multipotent pro-genitor cells, such as dedifferentiated fat (DFAT) cells, are prospective sources for regenerative therapies. Herein, we aimed to demonstrate that a composite gelatin sponge (α-TCP/GS) of al-fa-tricalcium phosphate (α-TCP) mixed with gelatin scaffolds (GS), with/without DFATs, induced bone regeneration in a rat calvarial defect model in vivo. α-TCP/GS was prepared by mixing α-TCP and 2% GS using vacuum-heated methods. α-TCP/GS samples with/without DFATs were transplanted into the model. After 4 weeks of implantation, the samples were subjected to mi-cro-computed tomography (μ-CT) and histological analysis. α-TCP/GS possessed adequate me-chanical strength; α-TCP did not convert to hydroxyapatite upon contact with water, as deter-mined by X-ray diffraction. Moreover, stable α-TCP/GS was formed by electrostatic interactions, and verified based on the infrared peak shifts. μ-CT analyses showed that bone formation was higher in the α-TCP/GS+ DFAT group than in the α-TCP/GS group. Therefore, the implantation of α-TCP/GS comprising DFAT cells enhanced bone regeneration and vascularization, demon-strating the potential for healing critical-sized bone defects.; 2021年度
• Access Restrictions: インターネット公開
• Rights (production): CC BY-NC-ND
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 大阪歯科大学 : 大阪歯科大学学術リポジトリ

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