NHE-1 blockade reversed changes in calcium transient in myocardial slices from isoproterenol-induced hypertrophied rat left ventricle.

• 資料種別: 博士論文
• タイトル: NHE-1 blockade reversed changes in calcium transient in myocardial slices from isoproterenol-induced hypertrophied rat left ventricle.
• 著者・編者: Hattori, Hiroshi; Takeshita, Daisuke; Takeuchi, Ayako; Kim, Bongju; Shibata, Munetaka; Matsuoka, Satoshi; Obata, Koji; Mitsuyama, Shinichi; Zhang, Guo-Xing; Takaki, Miyako
• 著者標目: Hattori, Hiroshi; Takeshita, Daisuke; Takeuchi, Ayako; Kim, Bongju; Shibata, Munetaka; Matsuoka, Satoshi; Obata, Koji; Mitsuyama, Shinichi; Zhang, Guo-Xing; Takaki, Miyako
• 出版事項: Academic Press / Elsevier
• 出版年月日等: 2012-03-09
• 出版年（W3CDTF）: 2012-03-09
• 並列タイトル等: イソプロテレノール誘導肥大心においてNHE-1阻害薬は、ラット左心室の筋スライスのCa2+トランジェントを正常化する
• タイトル（掲載誌）: Biochemical and biophysical research communications
• 巻号年月日等（掲載誌）: 419 2
• 掲載巻: 419
• 掲載号: 2
• 掲載ページ: 431-435
• ISSN（掲載誌）: ISSN : 0006-291X
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2014-03-17
• 報告番号: 甲第618号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: Calcium transient; Cardiac hypertrophy; Excitation–contraction coupling; Myocardial slice; Na+/H+ exchanger-1
• 対象利用者: 一般
• 一般注記: type:Thesis; We previously reported that left ventricular (LV) slices from isoproterenol (ISO)-induced hypertrophied rat hearts showed an increase of energy expenditure due to remodeling of Ca2+ handling in excitation–contraction coupling, i.e., suppressed SERCA2a activity and enhanced Na+/Ca2+exchanger-1 (NCX-1) activity. Na+/H+ exchanger-1 (NHE-1) inhibitor (NHEI) has been demonstrated to exert beneficial effects in the development of cardiac remodeling. We hypothesized that a novel NHE-1 selective inhibitor, BIIB723 prevents remodeling of Ca2+ handling in LV slices of ISO-induced hypertrophied rat hearts mediated by inhibiting NCX-1 activity. The significant shortening in duration of multi-cellular Ca2+ transient in ISO group was normalized in ISO + BIIB723 group. The significant increase in amplitude of multi-cellular Ca2+ waves (CaW) generated at high [Ca2+]o of LV slices in ISO group was also normalized in ISO + BIIB723 group. However, the enhanced NCX-1 activity was not antagonized by BIIB723. We recently reported that ISO-induced down-regulation of a Ca2+ handling protein, SERCA2a, was normalized by BIIB723. Therefore, it seems likely that BIIB723 normalized shortened multi-cellular Ca2+ transient duration and increased CaW amplitude in LV slices mediated via normalization of SERCA2a activity. Furthermore, the results presented here suggest the multi-cellular Ca2+ transient duration and CaW amplitude in LV slices might be better indices reflecting SERCA2a activity than SERCA2a protein expression level.; 博士（医学）・甲618号・平成26年3月17日; identifier:Biochemical and biophysical research communications Vol.419 No.2 p.431-435; identifier:0006291X; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/2703; identifier:Biochemical and biophysical research communications, 419(2): 431-435
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.1016/j.bbrc.2012.02.041
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

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http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/2703/8/01%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/2703/2/02%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/2703/3/03%e6%9c%ac%e6%96%87.pdf fulltext