NHE-1 blockade reversed changes in calcium transient in myocardial slices from isoproterenol-induced hypertrophied rat left ventricle.

• Material Type: 博士論文
• Title: NHE-1 blockade reversed changes in calcium transient in myocardial slices from isoproterenol-induced hypertrophied rat left ventricle.
• Author/Editor: Hattori, Hiroshi; Takeshita, Daisuke; Takeuchi, Ayako; Kim, Bongju; Shibata, Munetaka; Matsuoka, Satoshi; Obata, Koji; Mitsuyama, Shinichi; Zhang, Guo-Xing; Takaki, Miyako
• Author Heading: Hattori, Hiroshi; Takeshita, Daisuke; Takeuchi, Ayako; Kim, Bongju; Shibata, Munetaka; Matsuoka, Satoshi; Obata, Koji; Mitsuyama, Shinichi; Zhang, Guo-Xing; Takaki, Miyako
• Publication, Distribution, etc.: Academic Press / Elsevier
• Publication Date: 2012-03-09
• Publication Date (W3CDTF): 2012-03-09
• Alternative Title: イソプロテレノール誘導肥大心においてNHE-1阻害薬は、ラット左心室の筋スライスのCa2+トランジェントを正常化する
• Periodical title: Biochemical and biophysical research communications
• No. or year of volume/issue: 419 2
• Volume: 419
• Issue: 2
• Pages: 431-435
• ISSN (Periodical Title): ISSN : 0006-291X
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2014-03-17
• Dissertation Number: 甲第618号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Calcium transient; Cardiac hypertrophy; Excitation–contraction coupling; Myocardial slice; Na+/H+ exchanger-1
• Target Audience: 一般
• Note (General): type:Thesis; We previously reported that left ventricular (LV) slices from isoproterenol (ISO)-induced hypertrophied rat hearts showed an increase of energy expenditure due to remodeling of Ca2+ handling in excitation–contraction coupling, i.e., suppressed SERCA2a activity and enhanced Na+/Ca2+exchanger-1 (NCX-1) activity. Na+/H+ exchanger-1 (NHE-1) inhibitor (NHEI) has been demonstrated to exert beneficial effects in the development of cardiac remodeling. We hypothesized that a novel NHE-1 selective inhibitor, BIIB723 prevents remodeling of Ca2+ handling in LV slices of ISO-induced hypertrophied rat hearts mediated by inhibiting NCX-1 activity. The significant shortening in duration of multi-cellular Ca2+ transient in ISO group was normalized in ISO + BIIB723 group. The significant increase in amplitude of multi-cellular Ca2+ waves (CaW) generated at high [Ca2+]o of LV slices in ISO group was also normalized in ISO + BIIB723 group. However, the enhanced NCX-1 activity was not antagonized by BIIB723. We recently reported that ISO-induced down-regulation of a Ca2+ handling protein, SERCA2a, was normalized by BIIB723. Therefore, it seems likely that BIIB723 normalized shortened multi-cellular Ca2+ transient duration and increased CaW amplitude in LV slices mediated via normalization of SERCA2a activity. Furthermore, the results presented here suggest the multi-cellular Ca2+ transient duration and CaW amplitude in LV slices might be better indices reflecting SERCA2a activity than SERCA2a protein expression level.; 博士（医学）・甲618号・平成26年3月17日; identifier:Biochemical and biophysical research communications Vol.419 No.2 p.431-435; identifier:0006291X; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/2703; identifier:Biochemical and biophysical research communications, 419(2): 431-435
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1016/j.bbrc.2012.02.041
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

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