並列タイトル等自転−公転ミキサーを用いた人工赤血球(ヘモグロビン ベシクル)の高いカプセル化効率のための調製法
タイトル(掲載誌)ACS biomaterials science and engineering
一般注記type:Thesis
Hemoglobin vesicles (Hb-V) are artificial red blood cells encapsulating highly concentrated hemoglobin (Hb) in liposomes comprising phospholipids, cholesterol, negatively charged lipids, and polyethylene glycol (PEG)-conjugated phospholipids. Safety and efficacy of Hb-V as a transfusion alternative have been extensively studied. For this study, we prepared Hb-V using the kneading method with a rotation-revolution mixer as an alternative to the conventional extrusion method. We optimized the kneading operation parameters to obtain Hb-V with a high yield. Results show that the Hb encapsulation efficiency was increased dramatically up to 74.2%, which is higher than that of the extrusion method (20%) because the kneading method enabled mixing of a highly concentrated carbonylhemoglobin (HbCO) solution (40 g/dL) and a considerably large amount of powdered lipids in only 10 min. The high viscosity of the Hb-lipid mixture paste (ca. 103-105 cP) favorably induces frictional heat by kneading and increases the paste temperature (ca. 60 °C), which facilitates lipid dispersion and liposome formation. During the kneading operation using a thermostable HbCO solution, Hb denaturation was prevented. Hb-V prepared using this method showed no marked changes in particle sizes, Hb denaturation, or Hb leakage from liposomes during two years of long-term storage-stability tests. Collectively, these results demonstrate that the kneading method using a rotation-revolution mixer shows good potential as a new method to produce Hb-V.
博士(医学)・甲第797号・令和3年9月29日
This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS biomaterials science and engineering, copyright © 2021 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsbiomaterials.1c00424
identifier:ACS biomaterials science and engineering Vol.7 No.6 p.2835-2844 (2021 Jun)
identifier:23739878
identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3946
identifier:ACS biomaterials science and engineering, 7(6): 2835-2844
関連情報(DOI)10.1021/acsbiomaterials.1c00424
連携機関・データベース国立情報学研究所 : 学術機関リポジトリデータベース(IRDB)(機関リポジトリ)
提供元機関・データベース奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU