Preparation of Artificial Red Blood Cells (Hemoglobin Vesicles) Using the Rotation-Revolution Mixer for High Encapsulation Efficiency.

• Material Type: 博士論文
• Title: Preparation of Artificial Red Blood Cells (Hemoglobin Vesicles) Using the Rotation-Revolution Mixer for High Encapsulation Efficiency.
• Author/Editor: Kure, Tomoko; Sakai, Hiromi
• Author Heading: Kure, Tomoko; Sakai, Hiromi
• Publication, Distribution, etc.: American Chemical Society
• Publication Date: 2021-06
• Publication Date (W3CDTF): 2021-06
• Alternative Title: 自転−公転ミキサーを用いた人工赤血球（ヘモグロビン ベシクル）の高いカプセル化効率のための調製法
• Periodical title: ACS biomaterials science and engineering
• No. or year of volume/issue: 7 6
• Volume: 7
• Issue: 6
• Pages: 2835-2844
• ISSN (Periodical Title): ISSN : 2373-9878
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2021-09-29
• Dissertation Number: 甲第797号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: liposomes; hemoglobin; blood substitutes; homogenization; planetary mixer
• Target Audience: 一般
• Note (General): type:Thesis; Hemoglobin vesicles (Hb-V) are artificial red blood cells encapsulating highly concentrated hemoglobin (Hb) in liposomes comprising phospholipids, cholesterol, negatively charged lipids, and polyethylene glycol (PEG)-conjugated phospholipids. Safety and efficacy of Hb-V as a transfusion alternative have been extensively studied. For this study, we prepared Hb-V using the kneading method with a rotation-revolution mixer as an alternative to the conventional extrusion method. We optimized the kneading operation parameters to obtain Hb-V with a high yield. Results show that the Hb encapsulation efficiency was increased dramatically up to 74.2%, which is higher than that of the extrusion method (20%) because the kneading method enabled mixing of a highly concentrated carbonylhemoglobin (HbCO) solution (40 g/dL) and a considerably large amount of powdered lipids in only 10 min. The high viscosity of the Hb-lipid mixture paste (ca. 103-105 cP) favorably induces frictional heat by kneading and increases the paste temperature (ca. 60 °C), which facilitates lipid dispersion and liposome formation. During the kneading operation using a thermostable HbCO solution, Hb denaturation was prevented. Hb-V prepared using this method showed no marked changes in particle sizes, Hb denaturation, or Hb leakage from liposomes during two years of long-term storage-stability tests. Collectively, these results demonstrate that the kneading method using a rotation-revolution mixer shows good potential as a new method to produce Hb-V.; 博士（医学）・甲第797号・令和3年9月29日; This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS biomaterials science and engineering, copyright © 2021 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsbiomaterials.1c00424; identifier:ACS biomaterials science and engineering Vol.7 No.6 p.2835-2844 (2021 Jun); identifier:23739878; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3946; identifier:ACS biomaterials science and engineering, 7(6): 2835-2844
• Source: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3946/1/01%e7%94%b2797%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3946/2/02%e7%94%b2797%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3946/6/03%e7%94%b2797%e6%9c%ac%e6%96%87.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1021/acsbiomaterials.1c00424
• Periodical Title (NCID): AA12739394; https://ci.nii.ac.jp/ncid/AA12739394
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU