並列タイトル等脳皮質静脈梗塞ラットモデルを用いた脳静脈虚血による神経細胞および神経前駆細胞の発現と分布
タイトル(掲載誌)IBRO neuroscience reports
一般注記type:Thesis
Neurogenesis in the subventricular zone (SVZ), subgranular zone (SGZ), and cerebral cortex is now a familiar
event to confirm by cerebral arterial ischemia in rat models. However, it remains unclear whether cerebral
venous ischemia (CVI) alone causes neurogenesis, and where that neurogenesis occurs. After creating CVI rat
models via a two-vein occlusion (2-VO) method, neurogenesis was immunohistochemically evaluated by doublelabeling
5-bromo-2′ -deoxyuridine (BrdU)-positive cells with neuronal nuclei (NeuN) or doublecortin (DCX)
antibody. Fifty Wistar rats were divided into two major groups (BrdU-NeuN and BrdU-DCX) and then separated
into two subgroups (2-VO or sham). The total number of double-positive cells expressed inside a predefined
region of interest (ROI) covering the ischemic area was compared between the two subgroups. Then, we divided
the ROI into six sections to evaluate and compare the distribution of double-positive cells generated in each
section between the two subgroups. The 2-VO subgroup presented more double-positive cells than the sham
group in both BrdU-NeuN and BrdU-DCX groups, while the BrdU-DCX+2-VO group showed a characteristic
distribution of double-positive cells in ROI 2 and ROI 3, suggesting areas of the ischemic core and penumbra,
with a significant difference compared to the BrdU-DCX+sham group. This study demonstrates that CVI has the
potential to induce endogenous neurogenesis, with significant numbers of both newly generated neurons and
precursors observed in the ischemic area. The distribution of these cells suggests that the cortex could be the
main origin of neurogenesis after cortical CVI.
博士(医学)・甲第872号・令和5年3月15日
© 2022 The Authors. Published by Elsevier Ltd on behalf of International Brain Research Organization.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
identifier:IBRO neuroscience reports Vol.14 p.50-56 (2022 Dec)
identifier:26672421
identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/4104
identifier:IBRO neuroscience reports, 14: 50-56
関連情報(DOI)10.1016/j.ibneur.2022.12.005
連携機関・データベース国立情報学研究所 : 学術機関リポジトリデータベース(IRDB)(機関リポジトリ)
提供元機関・データベース奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU