Expression and distribution of generated neurons and endogenous precursors in rat cerebral cortical venous ischemia

• Material Type: 博士論文
• Title: Expression and distribution of generated neurons and endogenous precursors in rat cerebral cortical venous ischemia
• Author/Editor: Kim, Tae-Kyun; Takeshima, Yasuhiro; Ouji, Yukiteru; Nishimura, Fumihiko; Nakagawa, Ichiro; Young-Soo, Park; Yoshikawa, Masahide; Nakase, Hiroyuki
• Author Heading: Kim, Tae-Kyun; Takeshima, Yasuhiro; Ouji, Yukiteru; Nishimura, Fumihiko; Nakagawa, Ichiro; Young-Soo, Park; Yoshikawa, Masahide; Nakase, Hiroyuki
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2022-12-13
• Publication Date (W3CDTF): 2022-12-13
• Alternative Title: 脳皮質静脈梗塞ラットモデルを用いた脳静脈虚血による神経細胞および神経前駆細胞の発現と分布
• Periodical title: IBRO neuroscience reports
• No. or year of volume/issue: 14
• Volume: 14
• Pages: 50-56
• ISSN (Periodical Title): ISSN : 2667-2421
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2023-03-15
• Dissertation Number: 甲第872号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Cerebral ischemia; Cerebral venous infarction; Endogenous precursors; Neural stem cells; Neurogenesis
• Target Audience: 一般
• Note (General): type:Thesis; Neurogenesis in the subventricular zone (SVZ), subgranular zone (SGZ), and cerebral cortex is now a familiar event to confirm by cerebral arterial ischemia in rat models. However, it remains unclear whether cerebral venous ischemia (CVI) alone causes neurogenesis, and where that neurogenesis occurs. After creating CVI rat models via a two-vein occlusion (2-VO) method, neurogenesis was immunohistochemically evaluated by doublelabeling 5-bromo-2′ -deoxyuridine (BrdU)-positive cells with neuronal nuclei (NeuN) or doublecortin (DCX) antibody. Fifty Wistar rats were divided into two major groups (BrdU-NeuN and BrdU-DCX) and then separated into two subgroups (2-VO or sham). The total number of double-positive cells expressed inside a predefined region of interest (ROI) covering the ischemic area was compared between the two subgroups. Then, we divided the ROI into six sections to evaluate and compare the distribution of double-positive cells generated in each section between the two subgroups. The 2-VO subgroup presented more double-positive cells than the sham group in both BrdU-NeuN and BrdU-DCX groups, while the BrdU-DCX+2-VO group showed a characteristic distribution of double-positive cells in ROI 2 and ROI 3, suggesting areas of the ischemic core and penumbra, with a significant difference compared to the BrdU-DCX+sham group. This study demonstrates that CVI has the potential to induce endogenous neurogenesis, with significant numbers of both newly generated neurons and precursors observed in the ischemic area. The distribution of these cells suggests that the cortex could be the main origin of neurogenesis after cortical CVI.; 博士（医学）・甲第872号・令和5年3月15日; © 2022 The Authors. Published by Elsevier Ltd on behalf of International Brain Research Organization. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).; identifier:IBRO neuroscience reports Vol.14 p.50-56 (2022 Dec); identifier:26672421; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/4104; identifier:IBRO neuroscience reports, 14: 50-56
• Source: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4104/1/01%e7%94%b2872%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4104/2/02%e7%94%b2872%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4104/6/03%e7%94%b2872%e6%9c%ac%e6%96%87.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1016/j.ibneur.2022.12.005
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU