Culture of organoids with vestibular cell-derived factors promotes differentiation of embryonic stem cells into inner ear vestibular hair cells

• 資料種別: 博士論文
• タイトル: Culture of organoids with vestibular cell-derived factors promotes differentiation of embryonic stem cells into inner ear vestibular hair cells
• 著者・編者: Osaki, Daisuke; Ouji, Yukiteru; Sakagami, Masaharu; Kitamura, Tomotaka; Misu, Masayasu; Kitahara, Tadashi; Yoshikawa, Masahide
• 著者標目: Osaki, Daisuke; Ouji, Yukiteru; Sakagami, Masaharu; Kitamura, Tomotaka; Misu, Masayasu; Kitahara, Tadashi; Yoshikawa, Masahide
• 出版事項: Elsevier
• 出版年月日等: 2023-02
• 出版年（W3CDTF）: 2023
• 並列タイトル等: 内耳前庭細胞由来因子とオルガノイド培養を組み合わせたES細胞から内耳前庭有毛細胞への分化誘導
• タイトル（掲載誌）: Journal of bioscience and bioengineering
• 巻号年月日等（掲載誌）: 135 2
• 掲載巻: 135
• 掲載号: 2
• 掲載ページ: 143-150
• ISSN（掲載誌）: ISSN : 1389-1723
• 授与機関名: 奈良県立医科大学
• 報告番号: 甲第877号
• 学位: 博士（医学）
• 件名標目: Conditioned medium; Differentiation; Embryonic stem cells; Hair cells; Organoid; Vestibular
• 対象利用者: 一般
• 一般注記: 出版タイプ： VoR; type:Thesis; Vestibular hair cells (V-HCs) residing in the inner ear have important roles related to balance. Although differentiation of pluripotent stem cells into HCs has been shown, an effective method has yet to be established. We previously reported that use of vestibular cell-derived conditioned medium (V-CM) was helpful to induce embryonic stem (ES) cells to differentiate into V-HC-like cells in two-dimensional (2D) cultures of ES-derived embryoid bodies (EBs). In the present report, V-CM was used with three-dimensional (3D) cultures of EBs, which resulted in augmented expression of V-HC-related markers (Math1, Myosin6, Brn3c, Dnah5), but not of the cochlear HC-related marker Lmod3. Gene expression analyses of both 2D and 3D EBs cultured for two weeks revealed a greater level of augmented induction of HC-related markers in the 3D-cultured EBs. These results indicate that a 3D culture in combination with use of V-CM is an effective method for producing V-HCs.; 博士（医学）・甲第877号・令和5年3月15日; identifier:Journal of bioscience and bioengineering Vol.135 No.2 p.143-150 (2023 Feb); identifier:13891723; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/4109; identifier:Journal of bioscience and bioengineering, 135(2): 143-150
• 一次資料へのリンクURL: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4109/1/01%e7%94%b2877%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4109/2/02%e7%94%b2877%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4109/6/03%e7%94%b2877%e6%9c%ac%e6%96%87.pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.1016/j.jbiosc.2022.11.005; https://doi.org/10.1016/j.jbiosc.2022.11.005
• 掲載誌（NCID）: AA11307678; https://ci.nii.ac.jp/ncid/AA11307678
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU