Culture of organoids with vestibular cell-derived factors promotes differentiation of embryonic stem cells into inner ear vestibular hair cells

• Material Type: 博士論文
• Title: Culture of organoids with vestibular cell-derived factors promotes differentiation of embryonic stem cells into inner ear vestibular hair cells
• Author/Editor: Osaki, Daisuke; Ouji, Yukiteru; Sakagami, Masaharu; Kitamura, Tomotaka; Misu, Masayasu; Kitahara, Tadashi; Yoshikawa, Masahide
• Author Heading: Osaki, Daisuke; Ouji, Yukiteru; Sakagami, Masaharu; Kitamura, Tomotaka; Misu, Masayasu; Kitahara, Tadashi; Yoshikawa, Masahide
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2023-02
• Publication Date (W3CDTF): 2023-02
• Alternative Title: 内耳前庭細胞由来因子とオルガノイド培養を組み合わせたES細胞から内耳前庭有毛細胞への分化誘導
• Periodical title: Journal of bioscience and bioengineering
• No. or year of volume/issue: 135 2
• Volume: 135
• Issue: 2
• Pages: 143-150
• ISSN (Periodical Title): ISSN : 1389-1723
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2023-03-15
• Dissertation Number: 甲第877号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Conditioned medium; Differentiation; Embryonic stem cells; Hair cells; Organoid; Vestibular
• Target Audience: 一般
• Note (General): type:Thesis; Vestibular hair cells (V-HCs) residing in the inner ear have important roles related to balance. Although differentiation of pluripotent stem cells into HCs has been shown, an effective method has yet to be established. We previously reported that use of vestibular cell-derived conditioned medium (V-CM) was helpful to induce embryonic stem (ES) cells to differentiate into V-HC-like cells in two-dimensional (2D) cultures of ES-derived embryoid bodies (EBs). In the present report, V-CM was used with three-dimensional (3D) cultures of EBs, which resulted in augmented expression of V-HC-related markers (Math1, Myosin6, Brn3c, Dnah5), but not of the cochlear HC-related marker Lmod3. Gene expression analyses of both 2D and 3D EBs cultured for two weeks revealed a greater level of augmented induction of HC-related markers in the 3D-cultured EBs. These results indicate that a 3D culture in combination with use of V-CM is an effective method for producing V-HCs.; 博士（医学）・甲第877号・令和5年3月15日; identifier:Journal of bioscience and bioengineering Vol.135 No.2 p.143-150 (2023 Feb); identifier:13891723; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/4109; identifier:Journal of bioscience and bioengineering, 135(2): 143-150
• Source: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4109/1/01%e7%94%b2877%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4109/2/02%e7%94%b2877%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4109/6/03%e7%94%b2877%e6%9c%ac%e6%96%87.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1016/j.jbiosc.2022.11.005
• Periodical Title (NCID): AA11307678; https://ci.nii.ac.jp/ncid/AA11307678
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU