Brainstem Organoids From Human Pluripotent Stem Cells.

• 資料種別: 博士論文
• タイトル: Brainstem Organoids From Human Pluripotent Stem Cells.
• 著者・編者: Eura, Nobuyuki; Matsui, Takeshi K; Luginbühl, Joachim; Matsubayashi, Masaya; Nanaura, Hitoki; Shiota, Tomo; Kinugawa, Kaoru; Iguchi, Naohiko; Kiriyama, Takao; Zheng, Canbin; Kouno, Tsukasa; Lan, Yan Jun; Kongpracha, Pornparn; Wiriyasermkul, Pattama; Sakaguchi, Yoshihiko M; Nagata, Riko; Komeda, Tomoya; Morikawa, Naritaka; Kitayoshi, Fumika; Jong, Miyong; Kobashigawa, Shinko; Nakanishi, Mari; Hasegawa, Masatoshi; Saito, Yasuhiko; Shiromizu, Takashi; Nishimura, Yuhei; Kasai, Takahiko; Takeda, Maiko; Kobayashi, Hiroshi; Inagaki, Yusuke; Tanaka, Yasuhito; Makinodan, Manabu; Kishimoto, Toshifumi; Kuniyasu, Hiroki; Nagamori, Shushi; Muotri, Alysson R; Shin, Jay W; Sugie, Kazuma; Mori, Eiichiro
• 著者標目: Eura, Nobuyuki; Matsui, Takeshi K; Luginbühl, Joachim; Matsubayashi, Masaya; Nanaura, Hitoki; Shiota, Tomo; Kinugawa, Kaoru; Iguchi, Naohiko; Kiriyama, Takao; Zheng, Canbin; Kouno, Tsukasa; Lan, Yan Jun; Kongpracha, Pornparn; Wiriyasermkul, Pattama; Sakaguchi, Yoshihiko M; Nagata, Riko; Komeda, Tomoya; Morikawa, Naritaka; Kitayoshi, Fumika; Jong, Miyong; Kobashigawa, Shinko; Nakanishi, Mari; Hasegawa, Masatoshi; Saito, Yasuhiko; Shiromizu, Takashi; Nishimura, Yuhei; Kasai, Takahiko; Takeda, Maiko; Kobayashi, Hiroshi; Inagaki, Yusuke; Tanaka, Yasuhito; Makinodan, Manabu; Kishimoto, Toshifumi; Kuniyasu, Hiroki; Nagamori, Shushi; Muotri, Alysson R; Shin, Jay W; Sugie, Kazuma; Mori, Eiichiro
• 出版事項: Frontiers Media
• 出版年月日等: 2020-06-26
• 出版年（W3CDTF）: 2020-06-26
• 並列タイトル等: ヒト多能性幹細胞を用いた脳幹オルガノイドの開発
• タイトル（掲載誌）: Frontiers in neuroscience
• 巻号年月日等（掲載誌）: 14
• 掲載巻: 14
• 掲載ページ: Article No.538-
• ISSN（掲載誌）: ISSN : 1662-453X
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2020-12-24
• 報告番号: 乙第1479号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: brain organoids; brainstem; neural crest; midbrain; dopaminergic neurons; human pluripotent stem cells; melanocyte
• 対象利用者: 一般
• 一般注記: type:Thesis; The brainstem is a posterior region of the brain, composed of three parts, midbrain, pons, and medulla oblongata. It is critical in controlling heartbeat, blood pressure, and respiration, all of which are life-sustaining functions, and therefore, damages to or disorders of the brainstem can be lethal. Brain organoids derived from human pluripotent stem cells (hPSCs) recapitulate the course of human brain development and are expected to be useful for medical research on central nervous system disorders. However, existing organoid models are limited in the extent hPSCs recapitulate human brain development and hence are not able to fully elucidate the diseases affecting various components of the brain such as brainstem. Here, we developed a method to generate human brainstem organoids (hBSOs), containing midbrain/hindbrain progenitors, noradrenergic and cholinergic neurons, dopaminergic neurons, and neural crest lineage cells. Single-cell RNA sequence (scRNA-seq) analysis, together with evidence from proteomics and electrophysiology, revealed that the cellular population in these organoids was similar to that of the human brainstem, which raises the possibility of making use of hBSOs in investigating central nervous system disorders affecting brainstem and in efficient drug screenings.; 博士（医学）・乙第1479号・令和2年12月24日; Copyright © 2020 Eura, Matsui, Luginbühl, Matsubayashi, Nanaura, Shiota, Kinugawa, Iguchi, Kiriyama, Zheng, Kouno, Lan, Kongpracha, Wiriyasermkul, Sakaguchi, Nagata, Komeda, Morikawa, Kitayoshi, Jong, Kobashigawa, Nakanishi, Hasegawa, Saito, Shiromizu, Nishimura, Kasai, Takeda, Kobayashi, Inagaki, Tanaka, Makinodan, Kishimoto, Kuniyasu, Nagamori, Muotri, Shin, Sugie and Mori. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.; identifier:Frontiers in neuroscience Vol.14 Article No.538 (2020 Jun); identifier:1662453X; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3824; identifier:Frontiers in neuroscience, 14: Article No.538
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.3389/fnins.2020.00538
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

目次

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3824/1/01%e4%b9%991479%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3824/2/02%e4%b9%991479%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3824/3/03%e4%b9%991479%e6%9c%ac%e6%96%87.pdf fulltext