Brainstem Organoids From Human Pluripotent Stem Cells.

• Material Type: 博士論文
• Title: Brainstem Organoids From Human Pluripotent Stem Cells.
• Author/Editor: Eura, Nobuyuki; Matsui, Takeshi K; Luginbühl, Joachim; Matsubayashi, Masaya; Nanaura, Hitoki; Shiota, Tomo; Kinugawa, Kaoru; Iguchi, Naohiko; Kiriyama, Takao; Zheng, Canbin; Kouno, Tsukasa; Lan, Yan Jun; Kongpracha, Pornparn; Wiriyasermkul, Pattama; Sakaguchi, Yoshihiko M; Nagata, Riko; Komeda, Tomoya; Morikawa, Naritaka; Kitayoshi, Fumika; Jong, Miyong; Kobashigawa, Shinko; Nakanishi, Mari; Hasegawa, Masatoshi; Saito, Yasuhiko; Shiromizu, Takashi; Nishimura, Yuhei; Kasai, Takahiko; Takeda, Maiko; Kobayashi, Hiroshi; Inagaki, Yusuke; Tanaka, Yasuhito; Makinodan, Manabu; Kishimoto, Toshifumi; Kuniyasu, Hiroki; Nagamori, Shushi; Muotri, Alysson R; Shin, Jay W; Sugie, Kazuma; Mori, Eiichiro
• Author Heading: Eura, Nobuyuki; Matsui, Takeshi K; Luginbühl, Joachim; Matsubayashi, Masaya; Nanaura, Hitoki; Shiota, Tomo; Kinugawa, Kaoru; Iguchi, Naohiko; Kiriyama, Takao; Zheng, Canbin; Kouno, Tsukasa; Lan, Yan Jun; Kongpracha, Pornparn; Wiriyasermkul, Pattama; Sakaguchi, Yoshihiko M; Nagata, Riko; Komeda, Tomoya; Morikawa, Naritaka; Kitayoshi, Fumika; Jong, Miyong; Kobashigawa, Shinko; Nakanishi, Mari; Hasegawa, Masatoshi; Saito, Yasuhiko; Shiromizu, Takashi; Nishimura, Yuhei; Kasai, Takahiko; Takeda, Maiko; Kobayashi, Hiroshi; Inagaki, Yusuke; Tanaka, Yasuhito; Makinodan, Manabu; Kishimoto, Toshifumi; Kuniyasu, Hiroki; Nagamori, Shushi; Muotri, Alysson R; Shin, Jay W; Sugie, Kazuma; Mori, Eiichiro
• Publication, Distribution, etc.: Frontiers Media
• Publication Date: 2020-06-26
• Publication Date (W3CDTF): 2020-06-26
• Alternative Title: ヒト多能性幹細胞を用いた脳幹オルガノイドの開発
• Periodical title: Frontiers in neuroscience
• No. or year of volume/issue: 14
• Volume: 14
• Pages: Article No.538-
• ISSN (Periodical Title): ISSN : 1662-453X
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2020-12-24
• Dissertation Number: 乙第1479号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: brain organoids; brainstem; neural crest; midbrain; dopaminergic neurons; human pluripotent stem cells; melanocyte
• Target Audience: 一般
• Note (General): type:Thesis; The brainstem is a posterior region of the brain, composed of three parts, midbrain, pons, and medulla oblongata. It is critical in controlling heartbeat, blood pressure, and respiration, all of which are life-sustaining functions, and therefore, damages to or disorders of the brainstem can be lethal. Brain organoids derived from human pluripotent stem cells (hPSCs) recapitulate the course of human brain development and are expected to be useful for medical research on central nervous system disorders. However, existing organoid models are limited in the extent hPSCs recapitulate human brain development and hence are not able to fully elucidate the diseases affecting various components of the brain such as brainstem. Here, we developed a method to generate human brainstem organoids (hBSOs), containing midbrain/hindbrain progenitors, noradrenergic and cholinergic neurons, dopaminergic neurons, and neural crest lineage cells. Single-cell RNA sequence (scRNA-seq) analysis, together with evidence from proteomics and electrophysiology, revealed that the cellular population in these organoids was similar to that of the human brainstem, which raises the possibility of making use of hBSOs in investigating central nervous system disorders affecting brainstem and in efficient drug screenings.; 博士（医学）・乙第1479号・令和2年12月24日; Copyright © 2020 Eura, Matsui, Luginbühl, Matsubayashi, Nanaura, Shiota, Kinugawa, Iguchi, Kiriyama, Zheng, Kouno, Lan, Kongpracha, Wiriyasermkul, Sakaguchi, Nagata, Komeda, Morikawa, Kitayoshi, Jong, Kobashigawa, Nakanishi, Hasegawa, Saito, Shiromizu, Nishimura, Kasai, Takeda, Kobayashi, Inagaki, Tanaka, Makinodan, Kishimoto, Kuniyasu, Nagamori, Muotri, Shin, Sugie and Mori. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.; identifier:Frontiers in neuroscience Vol.14 Article No.538 (2020 Jun); identifier:1662453X; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3824; identifier:Frontiers in neuroscience, 14: Article No.538
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.3389/fnins.2020.00538
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

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http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3824/1/01%e4%b9%991479%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3824/2/02%e4%b9%991479%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3824/3/03%e4%b9%991479%e6%9c%ac%e6%96%87.pdf fulltext