Toxicity of Amphotericin B in Rabbit Corneal Epithelial Cells Stored in Optisol™-GS: Corneal Epithelial Cell Morphology and Migration

• 資料種別: 博士論文
• タイトル: Toxicity of Amphotericin B in Rabbit Corneal Epithelial Cells Stored in Optisol™-GS: Corneal Epithelial Cell Morphology and Migration
• 著者・編者: 原田, 康平
• 著者標目: 原田, 康平
• 出版事項: Taylor & Francis
• 出版年月日等: 2022-09-07
• 出版年（W3CDTF）: 2022-09-07
• 並列タイトル等: Optisol™-GSに保存されたウサギ角膜上皮細胞におけるアムホテリシンBの毒性：角膜上皮細胞の形態と遊走
• タイトル（掲載誌）: Current Eye Research
• 巻号年月日等（掲載誌）: 47 9
• 掲載巻: 47
• 掲載号: 9
• 掲載ページ: 1259-1265
• ISSN（掲載誌）: ISSN : 0271-3683
• 授与機関名: Nagasaki University (長崎大学)
• 授与年月日: 2022-09-07
• 報告番号: 甲医歯薬第1462号
• 学位: 博士(医学)
• 本文の言語コード: eng
• 件名標目: Amphotericin B (AmB); Optisol™-GS; corneal epithelial cells (CECs); morphology; epithelial migration
• 対象利用者: 一般
• 一般注記: Purpose: To evaluate the toxicity of Amphotericin B (AmB) in Optisol™-GS Corneal Storage Media (Bausch & Lomb) on corneal epithelial cell (CEC) morphology and migration ability.Methods: Sclerocorneal strips were removed from male Japanese white rabbits, and then stored at 4 °C in Optisol™-GS containing 0 µg/ml of AmB (control group) and 2.5, 5, 25, and 50 µg/ml of AmB (AmB groups; four eyes per group). After 7 days of storage, CEC morphology was evaluated by hematoxylin-eosin staining, immunohistochemical staining (ZO-1), and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Moreover, to evaluate CEC migration ability, three corneal blocks (6–8 × 3 mm each) from one preserved cornea were cultured for 24 h, and the area of CEC migration (2 mm at the central region) onto the stromal surface was then measured.Results: At 5, 25, and 50 µg/ml of AmB, deformation and vacuolation of CECs were observed in all preserved corneas. ZO-1 expression was significantly reduced in corneas preserved at AmB concentrations of 25 and 50 µg/ml. TUNEL Labeling Index was significantly increased at AmB concentrations of ≥5 µg/ml. CEC migration was inhibited in a dose-dependent manner at AmB concentrations of 25 and 50 µg/ml compared to the control group.Conclusions: The addition of AmB to Optisol™-GS can be toxic to CECs and inhibit their migration at a concentration of ≥5 µg/ml. AmB at a concentration of 2.5 µg/ml can be considered safe for the preservation of donor corneal tissue used in corneal epithelial transplantation surgery.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1462号 学位授与年月日:令和4年9月7日; Author: Kohei Harada, Hideki Fukuoka, Yuriko Ban, Yulia Aziza, Go Horiguchi, Hidetoshi Tanioka, Shigeru Kinoshita, Masafumi Uematsu, Takashi Kitaoka & Chie Sotozono; Citation: Current Eye Research, 47(9), pp. 1259-1265; 2022; identifier:Nagasaki University (長崎大学), 博士(医学) (2022-09-07)
• 一次資料へのリンクURL: fulltext; https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=27738&item_no=1&attribute_id=70&file_no=1
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: This is an Accepted Manuscript of an article published by Taylor & Francis in Current Eye Research on 27 Jun 2022, available online: http://www.tandfonline.com/10.1080/02713683.2022.2091147.
• 関連情報: http://hdl.handle.net/10069/00041828
• 関連情報（DOI）: 10.1080/02713683.2022.2091147
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 長崎大学 : 長崎大学学術研究成果リポジトリ