Toxicity of Amphotericin B in Rabbit Corneal Epithelial Cells Stored in Optisol™-GS: Corneal Epithelial Cell Morphology and Migration

• Material Type: 博士論文
• Title: Toxicity of Amphotericin B in Rabbit Corneal Epithelial Cells Stored in Optisol™-GS: Corneal Epithelial Cell Morphology and Migration
• Author/Editor: 原田, 康平
• Author Heading: 原田, 康平
• Publication, Distribution, etc.: Taylor & Francis
• Publication Date: 2022-09-07
• Publication Date (W3CDTF): 2022-09-07
• Alternative Title: Optisol™-GSに保存されたウサギ角膜上皮細胞におけるアムホテリシンBの毒性：角膜上皮細胞の形態と遊走
• Periodical title: Current Eye Research
• No. or year of volume/issue: 47 9
• Volume: 47
• Issue: 9
• Pages: 1259-1265
• ISSN (Periodical Title): ISSN : 0271-3683
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2022-09-07
• Dissertation Number: 甲医歯薬第1462号
• Degree Type: 博士(医学)
• Text Language Code: eng
• Subject Heading: Amphotericin B (AmB); Optisol™-GS; corneal epithelial cells (CECs); morphology; epithelial migration
• Target Audience: 一般
• Note (General): Purpose: To evaluate the toxicity of Amphotericin B (AmB) in Optisol™-GS Corneal Storage Media (Bausch & Lomb) on corneal epithelial cell (CEC) morphology and migration ability.Methods: Sclerocorneal strips were removed from male Japanese white rabbits, and then stored at 4 °C in Optisol™-GS containing 0 µg/ml of AmB (control group) and 2.5, 5, 25, and 50 µg/ml of AmB (AmB groups; four eyes per group). After 7 days of storage, CEC morphology was evaluated by hematoxylin-eosin staining, immunohistochemical staining (ZO-1), and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Moreover, to evaluate CEC migration ability, three corneal blocks (6–8 × 3 mm each) from one preserved cornea were cultured for 24 h, and the area of CEC migration (2 mm at the central region) onto the stromal surface was then measured.Results: At 5, 25, and 50 µg/ml of AmB, deformation and vacuolation of CECs were observed in all preserved corneas. ZO-1 expression was significantly reduced in corneas preserved at AmB concentrations of 25 and 50 µg/ml. TUNEL Labeling Index was significantly increased at AmB concentrations of ≥5 µg/ml. CEC migration was inhibited in a dose-dependent manner at AmB concentrations of 25 and 50 µg/ml compared to the control group.Conclusions: The addition of AmB to Optisol™-GS can be toxic to CECs and inhibit their migration at a concentration of ≥5 µg/ml. AmB at a concentration of 2.5 µg/ml can be considered safe for the preservation of donor corneal tissue used in corneal epithelial transplantation surgery.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1462号 学位授与年月日:令和4年9月7日; Author: Kohei Harada, Hideki Fukuoka, Yuriko Ban, Yulia Aziza, Go Horiguchi, Hidetoshi Tanioka, Shigeru Kinoshita, Masafumi Uematsu, Takashi Kitaoka & Chie Sotozono; Citation: Current Eye Research, 47(9), pp. 1259-1265; 2022; identifier:Nagasaki University (長崎大学), 博士(医学) (2022-09-07)
• Source: https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=27738&item_no=1&attribute_id=70&file_no=1 （fulltext）; https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=27738&item_no=1&attribute_id=70&file_no=1
• Access Restrictions: インターネット公開
• Rights (production): This is an Accepted Manuscript of an article published by Taylor & Francis in Current Eye Research on 27 Jun 2022, available online: http://www.tandfonline.com/10.1080/02713683.2022.2091147.
• Related Material: http://hdl.handle.net/10069/00041828
• Related Material (DOI): 10.1080/02713683.2022.2091147
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 長崎大学 : 長崎大学学術研究成果リポジトリ