TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis

• 資料種別: 博士論文
• ISSN: 1341-321X
• タイトル: TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis
• 巻次・部編番号: 23 1
• 著者・編者: 川元, 康嗣
• 著者標目: 川元, 康嗣
• 出版事項: Elsevier
• 出版年月日等: 2017-02-22
• 出版年（W3CDTF）: 2017-02-22
• 並列タイトル等: TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する
• タイトル（掲載誌）: Journal of Infection and Chemotherapy
• 授与機関名: Nagasaki University (長崎大学)
• 授与年月日: 2017-02-22
• 授与年月日（W3CDTF）: 2017-02-22
• 報告番号: 甲医歯薬第927号
• 学位: 博士(医学)
• 博論授与番号: 甲医歯薬第927号
• 本文の言語コード: eng
• 件名標目: Anti-TNF-α therapy; Cell death; Caspase 3; Caspase 7; Lung epithelial cells
• 対象利用者: 一般
• 一般注記: Background: TNF-α plays an important role in the pathogenesis of Legionella pneumophila (Lp)-induced pneumonia. Patients undergoing anti-TNF-α therapy are at an increased risk of Lp infection. Lp infects both phagocytic and non-phagocytic cells such as airway epithelial cells; however, the role of TNF-α in airway epithelial cells is unknown. Methods: Human airway epithelial cell line NCI-H292 was infected with Lp NUL1 strain. After infection, both intracellular growth of Lp and cell death were evaluated after treating the cells with or without TNF-α. Apoptosis was examined by performing activated caspase-3/7 staining and by using a pan-caspase inhibitor. Results: Lp infected and replicated in NCI-H292 cells in a time-dependent manner, and TNF-α treatment of Lp-infected NCI-H292 cells inhibited Lp replication. Inhibitory effects of TNF-α on Lp replication were suppressed after treatment with a TNF-α-neutralizing antibody. Lp infection increased extracellular lactate dehydrogenase levels and decreased the number of living cells. Increased number of Lp-infected NCI-H292 cells showed caspase-3/7 activation, indicating they underwent apoptosis. TNF-α treatment inhibited Lp replication by increasing the apoptosis of NCI-H292 cells. Conclusions: Thus, our results suggested that airway epithelial cells were involved in the pathogenesis of Lp infection and that TNF-α played a protective role by inhibiting the intracellular replication of Lp and by increasing the apoptosis of Lp-infected airway epithelial cells. However, Lp infection should be investigated further in patients undergoing anti-TNF-α therapy who develop pneumonia.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第927号 学位授与年月日:平成29年2月22日; Author: Yasuhide Kawamoto, Yoshitomo Morinaga, Yumiko Kimura, Norihito Kaku, Kosuke Kosai, Naoki Uno, Hiroo Hasegawa, Katsunori Yanagihara; Citation: Journal of Infection and Chemotherapy, 23(1), pp.51?55; 2017; Nagasaki University (長崎大学), 博士(医学) (2017-02-22)
• DOI: info:doi/10.1016/j.jiac.2016.09.010; https://doi.org/info:doi/10.1016/j.jiac.2016.09.010
• 国立国会図書館永続的識別子: info:ndljp/pid/10342761; https://dl.ndl.go.jp/pid/10342761
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2017-05-02T23:17:13+09:00
• 作成日（W3CDTF）: 2020-12-21
• 記録形式（IMT）: PDF
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://hdl.handle.net/10069/37166; https://nagasaki-u.repo.nii.ac.jp/records/3973
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

目次

2021-03-07 再収集