TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis

• Material Type: 博士論文
• ISSN: 1341-321X
• Title: TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis
• Volume: 23 1
• Author/Editor: 川元, 康嗣
• Author Heading: 川元, 康嗣
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2017-02-22
• Publication Date (W3CDTF): 2017-02-22
• Alternative Title: TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する
• Periodical title: Journal of Infection and Chemotherapy
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2017-02-22
• Date Granted (W3CDTF): 2017-02-22
• Dissertation Number: 甲医歯薬第927号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医歯薬第927号
• Text Language Code: eng
• Subject Heading: Anti-TNF-α therapy; Cell death; Caspase 3; Caspase 7; Lung epithelial cells
• Target Audience: 一般
• Note (General): Background: TNF-α plays an important role in the pathogenesis of Legionella pneumophila (Lp)-induced pneumonia. Patients undergoing anti-TNF-α therapy are at an increased risk of Lp infection. Lp infects both phagocytic and non-phagocytic cells such as airway epithelial cells; however, the role of TNF-α in airway epithelial cells is unknown. Methods: Human airway epithelial cell line NCI-H292 was infected with Lp NUL1 strain. After infection, both intracellular growth of Lp and cell death were evaluated after treating the cells with or without TNF-α. Apoptosis was examined by performing activated caspase-3/7 staining and by using a pan-caspase inhibitor. Results: Lp infected and replicated in NCI-H292 cells in a time-dependent manner, and TNF-α treatment of Lp-infected NCI-H292 cells inhibited Lp replication. Inhibitory effects of TNF-α on Lp replication were suppressed after treatment with a TNF-α-neutralizing antibody. Lp infection increased extracellular lactate dehydrogenase levels and decreased the number of living cells. Increased number of Lp-infected NCI-H292 cells showed caspase-3/7 activation, indicating they underwent apoptosis. TNF-α treatment inhibited Lp replication by increasing the apoptosis of NCI-H292 cells. Conclusions: Thus, our results suggested that airway epithelial cells were involved in the pathogenesis of Lp infection and that TNF-α played a protective role by inhibiting the intracellular replication of Lp and by increasing the apoptosis of Lp-infected airway epithelial cells. However, Lp infection should be investigated further in patients undergoing anti-TNF-α therapy who develop pneumonia.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第927号 学位授与年月日:平成29年2月22日; Author: Yasuhide Kawamoto, Yoshitomo Morinaga, Yumiko Kimura, Norihito Kaku, Kosuke Kosai, Naoki Uno, Hiroo Hasegawa, Katsunori Yanagihara; Citation: Journal of Infection and Chemotherapy, 23(1), pp.51?55; 2017; Nagasaki University (長崎大学), 博士(医学) (2017-02-22)
• DOI: info:doi/10.1016/j.jiac.2016.09.010; https://doi.org/info:doi/10.1016/j.jiac.2016.09.010
• Persistent ID (NDL): info:ndljp/pid/10342761; https://dl.ndl.go.jp/pid/10342761
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2017-05-02T23:17:13+09:00
• Date Created (W3CDTF): 2020-12-21
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10069/37166; https://nagasaki-u.repo.nii.ac.jp/records/3973
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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2021-03-07 再収集