Double-stranded DNA introduction into intact plants using peptide-DNA complexes

• 資料種別: 記事
• タイトル: Double-stranded DNA introduction into intact plants using peptide-DNA complexes
• 著者・編者: Manoj Lakshmanan; Takeshi Yoshizumi; Kumar Sudesh
• 出版事項: 日本 : 日本植物細胞分子生物学会
• 出版年月日等: 2015
• 出版年（W3CDTF）: 2015
• タイトル（掲載誌）: Plant biotechnology
• 巻号年月日等（掲載誌）: 32(1)
• 掲載巻: 32(1)
• ISSN（掲載誌）: 1347-6114
• ISSN-L（掲載誌）: 1342-4580
• 本文の言語コード: eng
• DOI: 10.5511/plantbiotechnology.14.1210b; https://doi.org/10.5511/plantbiotechnology.14.1210b
• 国立国会図書館永続的識別子: info:ndljp/pid/11000416; https://dl.ndl.go.jp/pid/11000416
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > 学術機関 > 学協会; https://dl.ndl.go.jp/collections/B00043
• 収集根拠: インターネット資料収集保存事業（WARP）
• 受理日（W3CDTF）: 2017-12-08T10:56:38+09:00
• 保存日（W3CDTF）: 2015-08-15
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 遠隔複写可否（NDL）: 不可
• 掲載誌（URI）: https://warp.da.ndl.go.jp/info:ndljp/pid/9483390/www.jspcmb.jp/journal/pb32_1.html
• 掲載誌（国立国会図書館永続的識別子）: info:ndljp/pid/11000411; https://dl.ndl.go.jp/pid/11000411
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• コレクション（個別）: 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > 学術機関 > 学協会; https://dl.ndl.go.jp/collections/B00043
• オンライン閲覧公開範囲: インターネット公開
• 遠隔複写可否（NDL）: 不可
• 所蔵機関: 国立国会図書館
• 請求記号: Z54-J126
• 関連情報（URI）: http://dl.ndl.go.jp/info:ndljp/pid/11000416
• 関連情報（国立国会図書館永続的識別子）: info:ndljp/pid/11000416; http://dl.ndl.go.jp/info:ndljp/pid/11000416
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• 書誌ID（NDLBibID）: 026292486; http://id.ndl.go.jp/bib/026292486
• 整理区分コード: 632

• 要約等: Introducing exogenous genes into plant cells is an essential technique in many fields in plant science and biotechnology. Despite their huge advantages, disadvantages of current transfection methods include the requirement of expensive equipment, risk of gene damage, low transformation efficiency, transgene size limitations, and limitations of applicable plant types. Recently developed peptide-based gene carriers can deliver plasmid and double-stranded RNA. However, the delivery of double-stranded DNA (dsDNA), specifically PCR products, has not been studied. As dsDNA is handled in several plant science labs, peptide-based gene carriers are expected to be applicable to dsDNA in addition to plasmid DNA and double-stranded RNA. Here, we demonstrate dsDNA introduction into intact <i>Nicotiana benthamiana</i> leaves by using an ionic complex of a fusion peptide comprising (KH)₉ and Bp100 with dsDNA encoding <i>Renilla</i> luciferase as a reporter gene. The buffer condition for the complex preparation and infiltration significantly affected the transfection efficiency; this is because the structure of the complex in various protonated conditions contributed to the transfection efficiency. Structures of the complex and peptide are key factors for improving the peptide-based gene delivery system for plants.
• DOI: 10.5511/plantbiotechnology.14.1210b
• オンライン閲覧公開範囲: インターネット公開
• 連携機関・データベース: 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp

• 要約等: Introducing exogenous genes into plant cells is an essential technique in many fields in plant science and biotechnology. Despite their huge advantages, disadvantages of current transfection methods include the requirement of expensive equipment, risk of gene damage, low transformation efficiency, transgene size limitations, and limitations of applicable plant types. Recently developed peptide-based gene carriers can deliver plasmid and double-stranded RNA. However, the delivery of double-stranded DNA (dsDNA), specifically PCR products, has not been studied. As dsDNA is handled in several plant science labs, peptide-based gene carriers are expected to be applicable to dsDNA in addition to plasmid DNA and double-stranded RNA. Here, we demonstrate dsDNA introduction into intact <i>Nicotiana benthamiana</i> leaves by using an ionic complex of a fusion peptide comprising (KH)₉ and Bp100 with dsDNA encoding <i>Renilla</i> luciferase as a reporter gene. The buffer condition for the complex preparation and infiltration significantly affected the transfection efficiency; this is because the structure of the complex in various protonated conditions contributed to the transfection efficiency. Structures of the complex and peptide are key factors for improving the peptide-based gene delivery system for plants.
• DOI: 10.5511/plantbiotechnology.14.1210b; https://doi.org/10.5511/plantbiotechnology.14.1210b
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（URI）: http://id.ndl.go.jp/bib/026292486; https://ndlsearch.ndl.go.jp/books/R000000004-I026292486; https://dl.ndl.go.jp/pid/11000416; http://id.ndl.go.jp/digimeta/11000416
• 参照: Plant Mitochondrial-Targeted Gene Delivery by Peptide/DNA Micelles Quantitatively Surface-Modified with Mitochondrial Targeting and Membrane-Penetrating Peptides; https://cir.nii.ac.jp/crid/1050016880901693568; Endosome-escaping micelle complexes dually equipped with cell-penetrating and endosome-disrupting peptides for efficient DNA delivery into intact plants; https://cir.nii.ac.jp/crid/1360290617744394624; Particle bombardment-assisted peptide-mediated gene transfer for highly efficient transient assay; https://cir.nii.ac.jp/crid/1360298754813829248; Fusion Peptide-Based Biomacromolecule Delivery System for Plant Cells; https://cir.nii.ac.jp/crid/1360576118770587136; Poly(amino acid)s/polypeptides as potential functional and structural materials; https://cir.nii.ac.jp/crid/1360845539041350272; Functional peptide-mediated plastid transformation in tobacco, rice, and kenaf; https://cir.nii.ac.jp/crid/1360861291387378048; Dual Peptide-Based Gene Delivery System for the Efficient Transfection of Plant Callus Cells; https://cir.nii.ac.jp/crid/1361131417891076224; A centrifugation-assisted peptide-mediated gene transfer method for high-throughput analyses; https://cir.nii.ac.jp/crid/1390001288143737216; Direct introduction of neomycin phosphotransferase II protein into apple leaves to confer kanamycin resistance; https://cir.nii.ac.jp/crid/1390282679303460096
• 参照: Quantized Folding of Plasmid DNA Condensed with Block Catiomer into Characteristic Rod Structures Promoting Transgene Efficacy; https://cir.nii.ac.jp/crid/1360285708189375232; Local gene silencing in plants via synthetic ds<scp>RNA</scp> and carrier peptide; https://cir.nii.ac.jp/crid/1360285710786376064; Transfection and expression of plasmid DNA in plant cells by an arginine‐rich intracellular delivery peptide without protoplast preparation; https://cir.nii.ac.jp/crid/1360292618503651840; Bioengineered silk protein-based gene delivery systems; https://cir.nii.ac.jp/crid/1360292619719120768; Adsorption and Hydrolysis Reactions of Poly(hydroxybutyric acid) Depolymerases Secreted from <i>Ralstonia</i> <i>pickettii</i> T1 and <i>Penicillium </i><i>funiculosum</i> onto Poly[(<i>R</i>)-3-hydroxybutyric acid]; https://cir.nii.ac.jp/crid/1360292621510414464; Silk‐Based Nanocomplexes with Tumor‐Homing Peptides for Tumor‐Specific Gene Delivery; https://cir.nii.ac.jp/crid/1360565164562467072; Rapid and Efficient Gene Delivery into Plant Cells Using Designed Peptide Carriers; https://cir.nii.ac.jp/crid/1360567183118526464; Co-polymer of histidine and lysine markedly enhances transfection efficiency of liposomes; https://cir.nii.ac.jp/crid/1360574095100081152; Adding diversity to plant transformation; https://cir.nii.ac.jp/crid/1360574095225913216; A library of linear undecapeptides with bactericidal activity against phytopathogenic bacteria; https://cir.nii.ac.jp/crid/1360855569574419584; <i>Arabidopsis</i> Nitric Oxide Synthase1 Is Targeted to Mitochondria and Protects against Oxidative Damage and Dark-Induced Senescence; https://cir.nii.ac.jp/crid/1360855569776764416; Silica breaks through in plants; https://cir.nii.ac.jp/crid/1360855570115459968; Spider Silk-Based Gene Carriers for Tumor Cell-Specific Delivery; https://cir.nii.ac.jp/crid/1361418520073002496; Silk-Based Gene Carriers with Cell Membrane Destabilizing Peptides; https://cir.nii.ac.jp/crid/1361699993775843584; Chloroplast Transformation in <i>Chlamydomonas</i> with High Velocity Microprojectiles; https://cir.nii.ac.jp/crid/1361981469976141952; Gene delivery mediated by recombinant silk proteins containing cationic and cell binding motifs; https://cir.nii.ac.jp/crid/1362262944192573824; Study of uptake of cell penetrating peptides and their cargoes in permeabilized wheat immature embryos; https://cir.nii.ac.jp/crid/1362544419834610176; Solid phase peptide synthesis utilizing 9‐fluorenylmethoxycarbonyl amino acids; https://cir.nii.ac.jp/crid/1362825894298348416; Cationic oligopeptide‐mediated delivery of dsRNA for post‐transcriptional gene silencing in plant cells; https://cir.nii.ac.jp/crid/1363107371253976448; Adsorption of Biopolyester Depolymerase on Silicon Wafer and Poly[(<i>R</i>)‐3‐hydroxybutyric acid] Single Crystal Revealed by Real‐Time AFM; https://cir.nii.ac.jp/crid/1363388844522760320; Silk-based delivery systems of bioactive molecules; https://cir.nii.ac.jp/crid/1363388845474708992; Agrobacterium tumefaciens transfers extremely long T-DNAs by a unidirectional mechanism; https://cir.nii.ac.jp/crid/1363670320670217344; Enzymatic Degradation Processes of Poly[(<i>R</i>)-3-hydroxybutyric acid] and Poly[(<i>R</i>)-3-hydroxybutyric acid-<i>c</i><i>o</i>-(<i>R</i>)-3-hydroxyvaleric acid] Single Crystals Revealed by Atomic Force Microscopy: Effects of Molecular Weight and Second-Monomer Composition on Erosion Rates; https://cir.nii.ac.jp/crid/1363670320807496064; Gene transfer to plants by diverse species of bacteria; https://cir.nii.ac.jp/crid/1364233268340329600
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: Japan Link Center; https://japanlinkcenter.org/top; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org; CiNii Articles; 科学研究費助成事業データベース; https://kaken.nii.ac.jp/ja
• 書誌ID（NDLBibID）: 026292486; http://id.ndl.go.jp/bib/026292486; 11000416; http://id.ndl.go.jp/bib/11000416
• NII論文ID: 130005061602