Double-stranded DNA introduction into intact plants using peptide-DNA complexes
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DOI[10.5511/plantbiotechnology.14.1210b]to the data of the same series
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- Material Type
- 記事
- Author/Editor
- Manoj LakshmananTakeshi YoshizumiKumar Sudesh
- Publication, Distribution, etc.
- Publication Date
- 2015
- Publication Date (W3CDTF)
- 2015
- Periodical title
- Plant biotechnology
- No. or year of volume/issue
- 32(1)
- Volume
- 32(1)
- ISSN (Periodical Title)
- 1347-6114
- ISSN-L (Periodical Title)
- 1342-4580
- Text Language Code
- eng
- DOI
- 10.5511/plantbiotechnology.14.1210b
- Persistent ID (NDL)
- info:ndljp/pid/11000416
- Collection
- Collection (Materials For Handicapped People:1)
- Collection (particular)
- 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > 学術機関 > 学協会
- Acquisition Basis
- インターネット資料収集保存事業（WARP）
- Date Accepted (W3CDTF)
- 2017-12-08T10:56:38+09:00
- Date Captured (W3CDTF)
- 2015-08-15
- Format (IMT)
- application/pdf
- Access Restrictions
- インターネット公開
- Availability of remote photoduplication service
- 不可
- Periodical Title (URI)
- Periodical Title (Persistent ID (NDL))
- info:ndljp/pid/11000411
- Data Provider (Database)
- 国立国会図書館 : 国立国会図書館デジタルコレクション
- Collection (particular)
- 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > 学術機関 > 学協会
- Access Restrictions
- インターネット公開
- Availability of remote photoduplication service
- 不可
- Holding library
- 国立国会図書館
- Call No.
- Z54-J126
- Related Material (URI)
- Related Material (Persistent ID (NDL))
- info:ndljp/pid/11000416
- Data Provider (Database)
- 国立国会図書館 : 国立国会図書館雑誌記事索引
- Bibliographic ID (NDL)
- 026292486
- Bibliographic Record Category (NDL)
- 632
- Summary, etc.
- Introducing exogenous genes into plant cells is an essential technique in many fields in plant science and biotechnology. Despite their huge advantages, disadvantages of current transfection methods include the requirement of expensive equipment, risk of gene damage, low transformation efficiency, transgene size limitations, and limitations of applicable plant types. Recently developed peptide-based gene carriers can deliver plasmid and double-stranded RNA. However, the delivery of double-stranded DNA (dsDNA), specifically PCR products, has not been studied. As dsDNA is handled in several plant science labs, peptide-based gene carriers are expected to be applicable to dsDNA in addition to plasmid DNA and double-stranded RNA. Here, we demonstrate dsDNA introduction into intact <i>Nicotiana benthamiana</i> leaves by using an ionic complex of a fusion peptide comprising (KH)<sub>9</sub> and Bp100 with dsDNA encoding <i>Renilla</i> luciferase as a reporter gene. The buffer condition for the complex preparation and infiltration significantly affected the transfection efficiency; this is because the structure of the complex in various protonated conditions contributed to the transfection efficiency. Structures of the complex and peptide are key factors for improving the peptide-based gene delivery system for plants.
- DOI
- 10.5511/plantbiotechnology.14.1210b
- Access Restrictions
- インターネット公開
- Data Provider (Database)
- 科学技術振興機構 : J-STAGE
- Summary, etc.
- Introducing exogenous genes into plant cells is an essential technique in many fields in plant science and biotechnology. Despite their huge advantages, disadvantages of current transfection methods include the requirement of expensive equipment, risk of gene damage, low transformation efficiency, transgene size limitations, and limitations of applicable plant types. Recently developed peptide-based gene carriers can deliver plasmid and double-stranded RNA. However, the delivery of double-stranded DNA (dsDNA), specifically PCR products, has not been studied. As dsDNA is handled in several plant science labs, peptide-based gene carriers are expected to be applicable to dsDNA in addition to plasmid DNA and double-stranded RNA. Here, we demonstrate dsDNA introduction into intact <i>Nicotiana benthamiana</i> leaves by using an ionic complex of a fusion peptide comprising (KH)<sub>9</sub> and Bp100 with dsDNA encoding <i>Renilla</i> luciferase as a reporter gene. The buffer condition for the complex preparation and infiltration significantly affected the transfection efficiency; this is because the structure of the complex in various protonated conditions contributed to the transfection efficiency. Structures of the complex and peptide are key factors for improving the peptide-based gene delivery system for plants.
- DOI
- 10.5511/plantbiotechnology.14.1210b
- Access Restrictions
- インターネット公開
- Related Material (URI)
- Is Referenced By
- Plant Mitochondrial-Targeted Gene Delivery by Peptide/DNA Micelles Quantitatively Surface-Modified with Mitochondrial Targeting and Membrane-Penetrating PeptidesEndosome-escaping micelle complexes dually equipped with cell-penetrating and endosome-disrupting peptides for efficient DNA delivery into intact plantsParticle bombardment-assisted peptide-mediated gene transfer for highly efficient transient assayFusion Peptide-Based Biomacromolecule Delivery System for Plant CellsPoly(amino acid)s/polypeptides as potential functional and structural materialsFunctional peptide-mediated plastid transformation in tobacco, rice, and kenafDual Peptide-Based Gene Delivery System for the Efficient Transfection of Plant Callus CellsA centrifugation-assisted peptide-mediated gene transfer method for high-throughput analysesDirect introduction of neomycin phosphotransferase II protein into apple leaves to confer kanamycin resistance
- References
- Quantized Folding of Plasmid DNA Condensed with Block Catiomer into Characteristic Rod Structures Promoting Transgene EfficacyLocal gene silencing in plants via synthetic ds<scp>RNA</scp> and carrier peptideTransfection and expression of plasmid DNA in plant cells by an arginine‐rich intracellular delivery peptide without protoplast preparationBioengineered silk protein-based gene delivery systemsAdsorption and Hydrolysis Reactions of Poly(hydroxybutyric acid) Depolymerases Secreted from <i>Ralstonia</i> <i>pickettii</i> T1 and <i>Penicillium </i><i>funiculosum</i> onto Poly[(<i>R</i>)-3-hydroxybutyric acid]Silk‐Based Nanocomplexes with Tumor‐Homing Peptides for Tumor‐Specific Gene DeliveryRapid and Efficient Gene Delivery into Plant Cells Using Designed Peptide CarriersCo-polymer of histidine and lysine markedly enhances transfection efficiency of liposomesAdding diversity to plant transformationA library of linear undecapeptides with bactericidal activity against phytopathogenic bacteria<i>Arabidopsis</i> Nitric Oxide Synthase1 Is Targeted to Mitochondria and Protects against Oxidative Damage and Dark-Induced SenescenceSilica breaks through in plantsSpider Silk-Based Gene Carriers for Tumor Cell-Specific DeliverySilk-Based Gene Carriers with Cell Membrane Destabilizing PeptidesChloroplast Transformation in <i>Chlamydomonas</i> with High Velocity MicroprojectilesGene delivery mediated by recombinant silk proteins containing cationic and cell binding motifsStudy of uptake of cell penetrating peptides and their cargoes in permeabilized wheat immature embryosSolid phase peptide synthesis utilizing 9‐fluorenylmethoxycarbonyl amino acidsCationic oligopeptide‐mediated delivery of dsRNA for post‐transcriptional gene silencing in plant cellsAdsorption of Biopolyester Depolymerase on Silicon Wafer and Poly[(<i>R</i>)‐3‐hydroxybutyric acid] Single Crystal Revealed by Real‐Time AFMSilk-based delivery systems of bioactive moleculesAgrobacterium tumefaciens transfers extremely long T-DNAs by a unidirectional mechanismEnzymatic Degradation Processes of Poly[(<i>R</i>)-3-hydroxybutyric acid] and Poly[(<i>R</i>)-3-hydroxybutyric acid-<i>c</i><i>o</i>-(<i>R</i>)-3-hydroxyvaleric acid] Single Crystals Revealed by Atomic Force Microscopy: Effects of Molecular Weight and Second-Monomer Composition on Erosion RatesGene transfer to plants by diverse species of bacteria
- Data Provider (Database)
- 国立情報学研究所 : CiNii Research
- Original Data Provider (Database)
- Japan Link Center雑誌記事索引データベース雑誌記事索引データベースCrossrefCiNii Articles科学研究費助成事業データベースCrossrefCrossrefCrossrefCrossrefCrossrefCrossrefCrossrefCrossrefCrossref
- Bibliographic ID (NDL)
- 02629248611000416
- NAID
- 130005061602