Cellular Tight Junctions Prevent Effective Campylobacter jejuni Invasion and Inflammatory Barrier Disruption Promoting Bacterial Invasion from Lateral Membrane in Polarized Intestinal Epithelial Cells

• 資料種別: 博士論文
• ISSN: 2235-2988
• タイトル: Cellular Tight Junctions Prevent Effective Campylobacter jejuni Invasion and Inflammatory Barrier Disruption Promoting Bacterial Invasion from Lateral Membrane in Polarized Intestinal Epithelial Cells
• 巻次・部編番号: 8
• 著者・編者: Hatayama, Sho; Shimohata, Takaaki; Amano, Sachie; Kido, Junko; Nguyen, Anh Q.; Sato, Yuri; Kanda, Yuna; Tentaku, Aya; Fukushima, Shiho; Nakahashi, Mutsumi; Uebansou, Takashi; Mawatari, Kazuaki; Takahashi, Akira
• 著者標目: Hatayama, Sho; Shimohata, Takaaki; Amano, Sachie; Kido, Junko; Nguyen, Anh Q.; Sato, Yuri; Kanda, Yuna; Tentaku, Aya; Fukushima, Shiho; Nakahashi, Mutsumi; Uebansou, Takashi; Mawatari, Kazuaki; Takahashi, Akira
• 出版事項: Frontiers Media S.A.
• 出版年月日等: 2018-01-30
• 出版年（W3CDTF）: 2018-01-30
• 並列タイトル等: タイトジャンクションは極性化腸管上皮細胞においてCampylobacter jejuniの細胞側面からの効率的な侵入を妨げ炎症によるバリアの破綻は菌の侵入を促進する; Tight Junctions Regulate C. jejuni Invasion; タイトジャンクションは極性化上皮細胞においてCampylobacter jejuniの細胞側面からの効率的な侵入を妨げ炎症によるバリアの破綻は菌の侵入を促進する
• タイトル（掲載誌）: Frontiers in Cellular and Infection Microbiology
• 授与機関名: 徳島大学
• 授与年月日: 2018-03-23
• 授与年月日（W3CDTF）: 2018-03-23
• 報告番号: 甲第3143号
• 学位: 博士（栄養学）
• 博論授与番号: 甲第3143号
• 本文の言語コード: eng
• 件名標目: Campylobacter jejuni; Tight Junctions (TJs); lipid rafts; cell invasion; lateral part of epithelial cells
• 対象利用者: 一般
• 一般注記: Campylobacter jejuni invasion is closely related to C. jejuni pathogenicity. The intestinal epithelium contains polarized epithelial cells that form tight junctions (TJs) to provide a physical barrier against bacterial invasion. Previous studies indicated that C. jejuni invasion of non-polarized cells involves several cellular features, including lipid rafts. However, the dynamics of C. jejuni invasion of polarized epithelial cells are not fully understood. Here we investigated the interaction between C. jejuni invasion and TJ formation to characterize the mechanism of C. jejuni invasion in polarized epithelial cells. In contrast to non-polarized epithelial cells, C. jejuni invasion was not affected by depletion of lipid rafts in polarized epithelial cells. However, depletion of lipid rafts significantly decreased C. jejuni invasion in TJ disrupted cells or basolateral infection and repair of cellular TJs suppressed lipid raft-mediated C. jejuni invasion in polarized epithelial cells. In addition, pro-inflammatory cytokine, TNF-a treatment that induce TJ disruption promote C. jejuni invasion and lipid rafts depletion significantly reduced C. jejuni invasion in TNF-a treated cells. These data demonstrated that TJs prevent C. jejuni invasion from the lateral side of epithelial cells, where they play a main part in bacterial invasion and suggest that C. jejuni invasion could be increased in inflammatory condition. Therefore, maintenance of TJs integrity should be considered important in the development of novel therapies for C. jejuni infection.
• DOI: info:doi/10.3389/fcimb.2018.00015; https://doi.org/info:doi/10.3389/fcimb.2018.00015
• 国立国会図書館永続的識別子: info:ndljp/pid/11116353; https://dl.ndl.go.jp/pid/11116353
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2018-07-03T21:49:10+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://repo.lib.tokushima-u.ac.jp/111607
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp