Cellular Tight Junctions Prevent Effective Campylobacter jejuni Invasion and Inflammatory Barrier Disruption Promoting Bacterial Invasion from Lateral Membrane in Polarized Intestinal Epithelial Cells

• Material Type: 博士論文
• ISSN: 2235-2988
• Title: Cellular Tight Junctions Prevent Effective Campylobacter jejuni Invasion and Inflammatory Barrier Disruption Promoting Bacterial Invasion from Lateral Membrane in Polarized Intestinal Epithelial Cells
• Volume: 8
• Author/Editor: Hatayama, Sho; Shimohata, Takaaki; Amano, Sachie; Kido, Junko; Nguyen, Anh Q.; Sato, Yuri; Kanda, Yuna; Tentaku, Aya; Fukushima, Shiho; Nakahashi, Mutsumi; Uebansou, Takashi; Mawatari, Kazuaki; Takahashi, Akira
• Author Heading: Hatayama, Sho; Shimohata, Takaaki; Amano, Sachie; Kido, Junko; Nguyen, Anh Q.; Sato, Yuri; Kanda, Yuna; Tentaku, Aya; Fukushima, Shiho; Nakahashi, Mutsumi; Uebansou, Takashi; Mawatari, Kazuaki; Takahashi, Akira
• Publication, Distribution, etc.: Frontiers Media S.A.
• Publication Date: 2018-01-30
• Publication Date (W3CDTF): 2018-01-30
• Alternative Title: タイトジャンクションは極性化腸管上皮細胞においてCampylobacter jejuniの細胞側面からの効率的な侵入を妨げ炎症によるバリアの破綻は菌の侵入を促進する; Tight Junctions Regulate C. jejuni Invasion; タイトジャンクションは極性化上皮細胞においてCampylobacter jejuniの細胞側面からの効率的な侵入を妨げ炎症によるバリアの破綻は菌の侵入を促進する
• Periodical title: Frontiers in Cellular and Infection Microbiology
• Degree grantor/type: 徳島大学
• Date Granted: 2018-03-23
• Date Granted (W3CDTF): 2018-03-23
• Dissertation Number: 甲第3143号
• Degree Type: 博士（栄養学）
• Conferring No. (Dissertation): 甲第3143号
• Text Language Code: eng
• Subject Heading: Campylobacter jejuni; Tight Junctions (TJs); lipid rafts; cell invasion; lateral part of epithelial cells
• Target Audience: 一般
• Note (General): Campylobacter jejuni invasion is closely related to C. jejuni pathogenicity. The intestinal epithelium contains polarized epithelial cells that form tight junctions (TJs) to provide a physical barrier against bacterial invasion. Previous studies indicated that C. jejuni invasion of non-polarized cells involves several cellular features, including lipid rafts. However, the dynamics of C. jejuni invasion of polarized epithelial cells are not fully understood. Here we investigated the interaction between C. jejuni invasion and TJ formation to characterize the mechanism of C. jejuni invasion in polarized epithelial cells. In contrast to non-polarized epithelial cells, C. jejuni invasion was not affected by depletion of lipid rafts in polarized epithelial cells. However, depletion of lipid rafts significantly decreased C. jejuni invasion in TJ disrupted cells or basolateral infection and repair of cellular TJs suppressed lipid raft-mediated C. jejuni invasion in polarized epithelial cells. In addition, pro-inflammatory cytokine, TNF-a treatment that induce TJ disruption promote C. jejuni invasion and lipid rafts depletion significantly reduced C. jejuni invasion in TNF-a treated cells. These data demonstrated that TJs prevent C. jejuni invasion from the lateral side of epithelial cells, where they play a main part in bacterial invasion and suggest that C. jejuni invasion could be increased in inflammatory condition. Therefore, maintenance of TJs integrity should be considered important in the development of novel therapies for C. jejuni infection.
• DOI: info:doi/10.3389/fcimb.2018.00015; https://doi.org/info:doi/10.3389/fcimb.2018.00015
• Persistent ID (NDL): info:ndljp/pid/11116353; https://dl.ndl.go.jp/pid/11116353
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2018-07-03T21:49:10+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/111607
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp