並列タイトル等Development of Reverse Transcription Loop-Mediated Isothermal Amplification for Simple and Rapid Detection of Promyelocytic Leukemia?Retinoic Acid Receptor α mRNA
一般注記【Background】 Acute promyelocytic leukemia (APL) is a disease characterized by expression of promyelocytic leukemia?retinoic acid receptor α (PML-RARα) chimeric mRNA. Although APL is curable, early death due to hemorrhage is a major problem. Here, we report the development of a simple and rapid diagnostic method for APL based on reverse transcription loop-mediated isothermal amplification (RT-LAMP). 【Methods】 An RT-LAMP primer set was designed to detect three types of PML-RARα mRNA in a single reaction. Serial dilutions of plasmid DNA containing bcr1, bcr2, or bcr3 PML-RARα sequences and RNA extracted from bone marrow aspirates of 6 patients with APL were used to compare the results of RT-LAMP and nested PCR assays. 【Results】 Plasmid DNA was amplified by RT-LAMP, for which the reaction time was > 4 h shorter and the lower detection limit was higher than for nested RTPCR. Six of 7 samples tested positive by both methods. 【Conclusion】 We developed an RT-LAMP assay for simple and rapid PML-RARα mRNA detection that may be clinically useful for point-of-care testing and APL diagnosis.
This research was published by Yonago Acta Medica: Yuki Hashimoto, Yuki Hatayama, Nao Kojima, Shota Morishita, Satoko Matsumoto, Yuzuru Hosoda, Ayako Hara and Toru Motokura. Development of Reverse Transcription Loop-Mediated Isothermal Amplification for
http://www.lib.tottori-u.ac.jp/yam/yam/yam59-4/59-4contents.html
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