前骨髄球性白血病レチノイン酸受容体αmRNAの簡便かつ迅速な検出のための逆転写ループ介在等温増幅法の構築

Persistent ID (NDL): info:ndljp/pid/11116664

Material type: 博士論文

Author: 橋本, 祐樹ほか

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Publication date: 2018-03-01

Material Format: Digital

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Name of awarding university/degree: 鳥取大学,博士(医学)

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Note (General)：: 【Background】 Acute promyelocytic leukemia (APL) is a disease characterized by expression of promyelocytic leukemia?retinoic acid receptor α (PML-RARα)...

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Material Type: 博士論文
Title: 前骨髄球性白血病レチノイン酸受容体αmRNAの簡便かつ迅速な検出のための逆転写ループ介在等温増幅法の構築
Author/Editor: 橋本, 祐樹
Hashimoto, Yuki
Author Heading: 橋本, 祐樹
Hashimoto, Yuki
Publication Date: 2018-03-01
Publication Date (W3CDTF): 2018-03-01
Alternative Title: Development of Reverse Transcription Loop-Mediated Isothermal Amplification for Simple and Rapid Detection of Promyelocytic Leukemia?Retinoic Acid Receptor α mRNA
Degree grantor/type: 鳥取大学
Date Granted: 2018-03-01
Date Granted (W3CDTF): 2018-03-01
Dissertation Number: 甲第873号
Degree Type: 博士(医学)
Conferring No. (Dissertation): 甲第873号
Text Language Code: eng
Subject Heading: acute promyelocytic leukemia
polymerase chain reaction
promyelocytic leukemia?retinoic acid receptor α
reverse transcription loop-mediated isothermal amplification
t(15;17) chromosome translocation
Target Audience: 一般
Note (General): 【Background】 Acute promyelocytic leukemia (APL) is a disease characterized by expression of promyelocytic leukemia?retinoic acid receptor α (PML-RARα) chimeric mRNA. Although APL is curable, early death due to hemorrhage is a major problem. Here, we report the development of a simple and rapid diagnostic method for APL based on reverse transcription loop-mediated isothermal amplification (RT-LAMP). 【Methods】 An RT-LAMP primer set was designed to detect three types of PML-RARα mRNA in a single reaction. Serial dilutions of plasmid DNA containing bcr1, bcr2, or bcr3 PML-RARα sequences and RNA extracted from bone marrow aspirates of 6 patients with APL were used to compare the results of RT-LAMP and nested PCR assays. 【Results】 Plasmid DNA was amplified by RT-LAMP, for which the reaction time was > 4 h shorter and the lower detection limit was higher than for nested RTPCR. Six of 7 samples tested positive by both methods. 【Conclusion】 We developed an RT-LAMP assay for simple and rapid PML-RARα mRNA detection that may be clinically useful for point-of-care testing and APL diagnosis.
This research was published by Yonago Acta Medica: Yuki Hashimoto, Yuki Hatayama, Nao Kojima, Shota Morishita, Satoko Matsumoto, Yuzuru Hosoda, Ayako Hara and Toru Motokura. Development of Reverse Transcription Loop-Mediated Isothermal Amplification for
http://www.lib.tottori-u.ac.jp/yam/yam/yam59-4/59-4contents.html
Persistent ID (NDL): info:ndljp/pid/11116664
https://dl.ndl.go.jp/pid/11116664
Collection: 障害者向け資料
Collection (Materials For Handicapped People:1): テキストデータ
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2018-07-03T21:49:10+09:00
Format (IMT): application/pdf
Access Restrictions: 国立国会図書館内限定公開
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Availability of remote photoduplication service: 可
Periodical Title (URI): https://repository.lib.tottori-u.ac.jp/records/5865
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