Evaluation of the inhibitory effect of ivermectin on the growth of Babesia and Theileria parasites in vitro and in vivo

• 資料種別: 記事
• タイトル: Evaluation of the inhibitory effect of ivermectin on the growth of Babesia and Theileria parasites in vitro and in vivo
• 著者・編者: Gaber El-Saber Batiha; Amani Magdy Beshbishy; Dickson Stuart Tayebwa
• 出版事項: 日本 : Springer Nature
• 出版年月日等: 2019-07-11
• 出版年（W3CDTF）: 2019-07-11
• タイトル（掲載誌）: Tropical medicine and health
• 巻号年月日等（掲載誌）: 47(42)
• 掲載巻: 47(42)
• ISSN（掲載誌）: 1349-4147
• ISSN-L（掲載誌）: 1348-8945
• 本文の言語コード: eng
• DOI: 10.1186/s41182-019-0171-8; https://doi.org/10.1186/s41182-019-0171-8
• 国立国会図書館永続的識別子: info:ndljp/pid/11377424; https://dl.ndl.go.jp/pid/11377424
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > その他; https://dl.ndl.go.jp/collections/B00035
• 収集根拠: オンライン資料収集制度
• 受理日（W3CDTF）: 2019-10-28T20:45:15+09:00
• 保存日（W3CDTF）: 2019-10-28
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: https://tropmedhealth.biomedcentral.com/articles
• 掲載誌（国立国会図書館永続的識別子）: info:ndljp/pid/11285294; https://dl.ndl.go.jp/pid/11285294
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• 要約等: application/pdf; Background: Treatment is the principle way to control and eliminate piroplasmosis. The search for new chemotherapy against Babesia and Theileria has become increasingly urgent due to parasite resistance to current drugs. Ivermectin (IVM) was the world's first endectocide, capable of killing a wide variety of parasites and vectors, both inside and outside the body. It is currently authorized to treat onchocerciasis, lymphatic filariasis, strongyloidiasis, and scabies. The current study documented the efficacy of IVM on the growth of Babesia and Theileria in vitro and in vivo. Methods: The fluorescence-based assay was used for evaluating the inhibitory effect of IVM on four Babesia species, including B. bovis, B. bigemina, B. divergens, B. caballi, and Theileria equi, the combination with diminazene aceturate (DA), clofazimine (CF), and atovaquone (AQ) on in vitro cultures, and on the multiplication of a B. microti-infected mouse model. The cytotoxicity of compounds was tested on Madin-Darby bovine kidney (MDBK), mouse embryonic fibroblast (NIH/3 T3), and human foreskin fibroblast (HFF) cell lines. Results: The half-maximal inhibitory concentration (IC50) values determined for IVM against B. bovis, B. bigemina, B. divergens, B. caballi, and T. equi were 53.3 ± 4.8, 98.6 ± 5.7, 30.1 ± 2.2, 43.7 ± 3.7, and 90.1 ± 8.1 μM, respectively. Toxicity assays on MDBK, NIH/3 T3, and HFF cell lines showed that IVM affected the viability of cells with a half-maximal effective concentration (EC50) of 138.9 ± 4.9, 283.8 ± 3.6, and 287.5 ± 7.6 μM, respectively. In the in vivo experiment, IVM, when administered intraperitoneally at 4 mg/kg, significantly (p < 0.05) inhibited the growth of B. microti in mice by 63%. Furthermore, combination therapies of IVM-DA, IVM-AQ, and IVM-CF at a half dose reduced the peak parasitemia of B. microti by 83.7%, 76.5%, and 74.4%, respectively. Moreover, this study confirmed the absence of B. microti DNA in groups treated with combination chemotherapy of IVM + DA and IVM + AQ 49 days after infection. Conclusions: These findings suggest that IVM has the potential to be an alternative remedy for treating piroplasmosis. © 2019 The Author(s).
• DOI: 10.1186/s41182-019-0171-8; https://doi.org/10.1186/s41182-019-0171-8; 10.60692/80076-md631; https://doi.org/10.60692/80076-md631; 10.60692/sprrc-drr55; https://doi.org/10.60692/sprrc-drr55
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: This is an open access article licensed under the terms of the Creative Commons Attribution (by) License. <http://creativecommons.org/lisenses/by/4.1/>
• 関連情報（URI）: https://obihiro.repo.nii.ac.jp/records/4440; http://id.ndl.go.jp/digimeta/11377424; https://dl.ndl.go.jp/pid/11377424; http://link.springer.com/content/pdf/10.1186/s41182-019-0171-8.pdf; http://link.springer.com/article/10.1186/s41182-019-0171-8/fulltext.html; https://search.jamas.or.jp/link/ui/2020369363
• 参照: Safety and efficacy of hydroxyurea and eflornithine against most blood parasites Babesia and Theileria; https://cir.nii.ac.jp/crid/1361413117384534016; Phytochemical Characterization and Chemotherapeutic Potential of Cinnamomum verum Extracts on the Multiplication of Protozoan Parasites In Vitro and In Vivo; https://cir.nii.ac.jp/crid/1362820494089017856; Therapeutic Effects of Atranorin towards the Proliferation of Babesia and Theileria Parasites; https://cir.nii.ac.jp/crid/1364227868976126208
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: 学術機関リポジトリデータベース; https://irdb.nii.ac.jp; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org; CiNii Articles
• 書誌ID（NDLBibID）: 11377424; http://id.ndl.go.jp/bib/11377424
• NII論文ID: 120006727119