Evaluation of the inhibitory effect of ivermectin on the growth of Babesia and Theileria parasites in vitro and in vivo

• Material Type: 記事
• Title: Evaluation of the inhibitory effect of ivermectin on the growth of Babesia and Theileria parasites in vitro and in vivo
• Author/Editor: Gaber El-Saber Batiha; Amani Magdy Beshbishy; Dickson Stuart Tayebwa
• Publication, Distribution, etc.: 日本 : Springer Nature
• Publication Date: 2019-07-11
• Publication Date (W3CDTF): 2019-07-11
• Periodical title: Tropical medicine and health
• No. or year of volume/issue: 47(42)
• Volume: 47(42)
• ISSN (Periodical Title): 1349-4147
• ISSN-L (Periodical Title): 1348-8945
• Text Language Code: eng
• DOI: 10.1186/s41182-019-0171-8; https://doi.org/10.1186/s41182-019-0171-8
• Persistent ID (NDL): info:ndljp/pid/11377424; https://dl.ndl.go.jp/pid/11377424
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > その他; https://dl.ndl.go.jp/collections/B00035
• Acquisition Basis: オンライン資料収集制度
• Date Accepted (W3CDTF): 2019-10-28T20:45:15+09:00
• Date Captured (W3CDTF): 2019-10-28
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Periodical Title (URI): https://tropmedhealth.biomedcentral.com/articles
• Periodical Title (Persistent ID (NDL)): info:ndljp/pid/11285294; https://dl.ndl.go.jp/pid/11285294
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• Summary, etc.: application/pdf; Background: Treatment is the principle way to control and eliminate piroplasmosis. The search for new chemotherapy against Babesia and Theileria has become increasingly urgent due to parasite resistance to current drugs. Ivermectin (IVM) was the world's first endectocide, capable of killing a wide variety of parasites and vectors, both inside and outside the body. It is currently authorized to treat onchocerciasis, lymphatic filariasis, strongyloidiasis, and scabies. The current study documented the efficacy of IVM on the growth of Babesia and Theileria in vitro and in vivo. Methods: The fluorescence-based assay was used for evaluating the inhibitory effect of IVM on four Babesia species, including B. bovis, B. bigemina, B. divergens, B. caballi, and Theileria equi, the combination with diminazene aceturate (DA), clofazimine (CF), and atovaquone (AQ) on in vitro cultures, and on the multiplication of a B. microti-infected mouse model. The cytotoxicity of compounds was tested on Madin-Darby bovine kidney (MDBK), mouse embryonic fibroblast (NIH/3 T3), and human foreskin fibroblast (HFF) cell lines. Results: The half-maximal inhibitory concentration (IC50) values determined for IVM against B. bovis, B. bigemina, B. divergens, B. caballi, and T. equi were 53.3 ± 4.8, 98.6 ± 5.7, 30.1 ± 2.2, 43.7 ± 3.7, and 90.1 ± 8.1 μM, respectively. Toxicity assays on MDBK, NIH/3 T3, and HFF cell lines showed that IVM affected the viability of cells with a half-maximal effective concentration (EC50) of 138.9 ± 4.9, 283.8 ± 3.6, and 287.5 ± 7.6 μM, respectively. In the in vivo experiment, IVM, when administered intraperitoneally at 4 mg/kg, significantly (p < 0.05) inhibited the growth of B. microti in mice by 63%. Furthermore, combination therapies of IVM-DA, IVM-AQ, and IVM-CF at a half dose reduced the peak parasitemia of B. microti by 83.7%, 76.5%, and 74.4%, respectively. Moreover, this study confirmed the absence of B. microti DNA in groups treated with combination chemotherapy of IVM + DA and IVM + AQ 49 days after infection. Conclusions: These findings suggest that IVM has the potential to be an alternative remedy for treating piroplasmosis. © 2019 The Author(s).
• DOI: 10.1186/s41182-019-0171-8; https://doi.org/10.1186/s41182-019-0171-8; 10.60692/80076-md631; https://doi.org/10.60692/80076-md631; 10.60692/sprrc-drr55; https://doi.org/10.60692/sprrc-drr55
• Access Restrictions: インターネット公開
• Rights (production): This is an open access article licensed under the terms of the Creative Commons Attribution (by) License. <http://creativecommons.org/lisenses/by/4.1/>
• Related Material (URI): https://obihiro.repo.nii.ac.jp/records/4440; http://id.ndl.go.jp/digimeta/11377424; https://dl.ndl.go.jp/pid/11377424; http://link.springer.com/content/pdf/10.1186/s41182-019-0171-8.pdf; http://link.springer.com/article/10.1186/s41182-019-0171-8/fulltext.html; https://search.jamas.or.jp/link/ui/2020369363
• Is Referenced By: Safety and efficacy of hydroxyurea and eflornithine against most blood parasites Babesia and Theileria; https://cir.nii.ac.jp/crid/1361413117384534016; Phytochemical Characterization and Chemotherapeutic Potential of Cinnamomum verum Extracts on the Multiplication of Protozoan Parasites In Vitro and In Vivo; https://cir.nii.ac.jp/crid/1362820494089017856; Therapeutic Effects of Atranorin towards the Proliferation of Babesia and Theileria Parasites; https://cir.nii.ac.jp/crid/1364227868976126208
• Data Provider (Database): 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• Original Data Provider (Database): 学術機関リポジトリデータベース; https://irdb.nii.ac.jp; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org; CiNii Articles
• Bibliographic ID (NDL): 11377424; http://id.ndl.go.jp/bib/11377424
• NAID: 120006727119