EFFECT OF A NANO-SCALE FINE HOLE PATTERN ON THE DIFFERENTIATION OF RAW264.7 CELLS INTO OSTEOCLASTS

• 資料種別: 博士論文
• タイトル: EFFECT OF A NANO-SCALE FINE HOLE PATTERN ON THE DIFFERENTIATION OF RAW264.7 CELLS INTO OSTEOCLASTS
• 著者・編者: 高田, 亮
• 著者標目: 高田, 亮
• 出版事項: Hokkaido University
• 出版年月日等: 2019-03-25
• 出版年（W3CDTF）: 2019-03-25
• 並列タイトル等: RAW264.7 細胞の破骨細胞への分化におよぼすナノスケール HOLE パターンの影響
• 寄与者: 吉田, 靖弘; 土門, 卓文; 佐藤, 嘉晃
• 授与機関名: 北海道大学
• 授与年月日: 2019-03-25
• 授与年月日（W3CDTF）: 2019-03-25
• 報告番号: 甲第13473号
• 学位: 博士(歯学)
• 博論授与番号: 甲第13473号
• 本文の言語コード: eng
• 件名標目: Cell attachment; differentiation; nano-pattern; hole pattern; RAW264.7 cells; osteoclasts
• NDC: 497
• 対象利用者: 一般
• 一般注記: Osteoclasts are associated with certain diseases such as osteopetrosis. The development and functions of osteoclasts are controlled by the micro- and nano-surface roughness of biomaterials. However, the effect of fine micro/nano-patterns on osteoclastic differentiation remains poorly understood. In the present study, we investigated the effect of fine shape patterns at the nano-scale on osteoclastic differentiation. A hole pattern of 500 nm in diameter and 500 nm in height was prepared using nanoimprinting of a glycol-modified polyethylene terephthalate (G-PET) film for a basic topological study. RAW264.7 cells were used as an alternative basic model to primary cells. The result of a cell attachment assay showed that the average number of attached cells on the hole patterned-film was 1.5 times higher than that on the planar film. A cell differentiation assay showed that the average number of multinucleated osteoclasts on the hole patterned-film was approximately 1.9 times higher than that on the planar film. However, the patterning effects in this study were quite small (p > 0.05). Although the morphology of the cells was basically similar when observed under an optical microscope, the morphologies of the attached and differentiated cells were slightly different in terms of their cell shape and filopodia shape between the holed and planar films when observed under a scanning electron microscope. Differences in cell attachment and differentiation behaviors between holed and planar films would affect the functions of the osteoclasts. We suggest that fine nano-patterning of the substrate could be one factor to control osteoclastic differentiation and cell function.; (主査) 教授 吉田 靖弘, 教授 土門 卓文, 准教授 佐藤 嘉晃; 歯学研究科(口腔医学専攻)
• DOI: 10.14943/doctoral.k13473; https://doi.org/10.14943/doctoral.k13473
• 国立国会図書館永続的識別子: info:ndljp/pid/11479170; https://dl.ndl.go.jp/pid/11479170
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2020-04-06T03:04:11+09:00
• 記録形式（IMT）: PDF
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://dx.doi.org/10.14943/doctoral.k13473; http://hdl.handle.net/2115/77061
• 参照（URI）: http://hdl.handle.net/2115/73972
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• 要約等: Osteoclasts are associated with certain diseases such as osteopetrosis. The development and functions of osteoclasts are controlled by the micro- and ...