RAW264.7 細胞の破骨細胞への分化におよぼすナノスケール HOLE パターンの影響

• Material Type: 博士論文
• Title: RAW264.7 細胞の破骨細胞への分化におよぼすナノスケール HOLE パターンの影響
• Author/Editor: 高田, 亮
• Author Heading: 高田, 亮
• Publication, Distribution, etc.: Hokkaido University
• Publication Date: 2019-03-25
• Publication Date (W3CDTF): 2019-03-25
• Alternative Title: EFFECT OF A NANO-SCALE FINE HOLE PATTERN ON THE DIFFERENTIATION OF RAW264.7 CELLS INTO OSTEOCLASTS
• Contributor: 吉田, 靖弘; 土門, 卓文; 佐藤, 嘉晃
• Degree Grantor: 北海道大学; Hokkaido University
• Date Granted: 2019-03-25
• Date Granted (W3CDTF): 2019-03-25
• Dissertation Number: 甲第13473号
• Degree Type: 博士(歯学)
• Conferring No. (Dissertation): 甲第13473号
• Text Language Code: eng
• Subject Heading: Cell attachment; differentiation; nano-pattern; hole pattern; RAW264.7 cells; osteoclasts
• NDC: 497
• Target Audience: 一般
• Note (General): (主査) 教授 吉田 靖弘, 教授 土門 卓文, 准教授 佐藤 嘉晃; 歯学研究科(口腔医学専攻)
• DOI: 10.14943/doctoral.k13473; https://doi.org/10.14943/doctoral.k13473
• Persistent ID (NDL): info:ndljp/pid/11479170; https://dl.ndl.go.jp/pid/11479170
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-04-06T03:04:11+09:00
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): https://doi.org/10.14943/doctoral.k13473; http://hdl.handle.net/2115/77061
• Is Referenced By (URI): http://hdl.handle.net/2115/73972
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• Summary, etc.: Osteoclasts are associated with certain diseases such as osteopetrosis. The development and functions of osteoclasts are controlled by the micro- and nano-surface roughness of biomaterials. However, the effect of fine micro/nano-patterns on osteoclastic differentiation remains poorly understood. In the present study, we investigated the effect of fine shape patterns at the nano-scale on osteoclastic differentiation. A hole pattern of 500 nm in diameter and 500 nm in height was prepared using nanoimprinting of a glycol-modified polyethylene terephthalate (G-PET) film for a basic topological study. RAW264.7 cells were used as an alternative basic model to primary cells. The result of a cell attachment assay showed that the average number of attached cells on the hole patterned-film was 1.5 times higher than that on the planar film. A cell differentiation assay showed that the average number of multinucleated osteoclasts on the hole patterned-film was approximately 1.9 times higher than that on the planar film. However, the patterning effects in this study were quite small (p > 0.05). Although the morphology of the cells was basically similar when observed under an optical microscope, the morphologies of the attached and differentiated cells were slightly different in terms of their cell shape and filopodia shape between the holed and planar films when observed under a scanning electron microscope. Differences in cell attachment and differentiation behaviors between holed and planar films would affect the functions of the osteoclasts. We suggest that fine nano-patterning of the substrate could be one factor to control osteoclastic differentiation and cell function.
• DOI: 10.14943/doctoral.k13473; https://doi.org/10.14943/doctoral.k13473
• Format (IMT): application/pdf
• Source: Ryo_Takata.pdf （fulltext）; https://eprints.lib.hokudai.ac.jp/repo/huscap/all/77061/Ryo_Takata.pdf
• Access Restrictions: インターネット公開
• Is Referenced By (URI): HDL; https://hdl.handle.net/2115/73972
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 北海道大学 : 北海道大学学術成果コレクション