並列タイトル等マイクロスケールパターン上におけるRAW264.7細胞の融合
一般注記Macrophage-dependent immune response to the implant surface sometimes results in failure of implant. Macrophage behaviors are sensitive to the topological factors involving micro/nano-patterns on the implant surface. However, further investigations about macrophage behaviors toward the fine micro/nano-patterns on the implant surface are required. In the present study, we investigated the effects of fine micro-scale pillar patterns found on the surface of implants on macrophage fusion. Micropillars with diameters of 2 μm, 1 μm, and 500 nm and a height of 500 nm were fabricated by nanoimprinting on a cyclo-olefin polymer (COP) film. RAW 264.7 macrophages attachment assay shows that 1 μm- and 500 nm-pillars improved cell attachment than the planar surface. Macrophage fusion assay shows that the number of multinucleated cells on 1 μm- and 500 nm-pillars was approximately 1.4 to 1.5 times higher than that on the planar surface. However, 2 μm-pillar was ineffective in both the assays. Interestingly, the expression of F-actin and vinculin in mononuclear and multinucleated cells on the pillars appeared ring shaped along the circumference of pillars, indicating frustrated phagocytosis. We suggest that fine micro-patterning of the substrate could be one of the factors that control cell attachment and fusion of RAW 264.7 cells.
(主査) 教授 吉田 靖弘, 教授 網塚 憲生, 准教授 佐藤 嘉晃
歯学研究科(口腔医学専攻)
コレクション(個別)国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
受理日(W3CDTF)2020-04-06T03:04:11+09:00
連携機関・データベース国立国会図書館 : 国立国会図書館デジタルコレクション