FUSION OF RAW 264.7 MACROPHAGE CELLS ON MICRO-SCALE FINE PILLAR PATTERNS

• Material Type: 博士論文
• Title: FUSION OF RAW 264.7 MACROPHAGE CELLS ON MICRO-SCALE FINE PILLAR PATTERNS
• Author/Editor: 本間, 淳
• Author Heading: 本間, 淳
• Publication, Distribution, etc.: Hokkaido University
• Publication Date: 2019-03-25
• Publication Date (W3CDTF): 2019-03-25
• Alternative Title: マイクロスケールパターン上におけるRAW264.7細胞の融合
• Contributor: 吉田, 靖弘; 網塚, 憲生; 佐藤, 嘉晃
• Degree grantor/type: 北海道大学
• Date Granted: 2019-03-25
• Date Granted (W3CDTF): 2019-03-25
• Dissertation Number: 甲第13477号
• Degree Type: 博士(歯学)
• Conferring No. (Dissertation): 甲第13477号
• Text Language Code: eng
• Subject Heading: Cell attachment; macrophage fusion; micro-pattern; pillar-pattern; RAW 264.7
• NDC: 497
• Target Audience: 一般
• Note (General): Macrophage-dependent immune response to the implant surface sometimes results in failure of implant. Macrophage behaviors are sensitive to the topological factors involving micro/nano-patterns on the implant surface. However, further investigations about macrophage behaviors toward the fine micro/nano-patterns on the implant surface are required. In the present study, we investigated the effects of fine micro-scale pillar patterns found on the surface of implants on macrophage fusion. Micropillars with diameters of 2 μm, 1 μm, and 500 nm and a height of 500 nm were fabricated by nanoimprinting on a cyclo-olefin polymer (COP) film. RAW 264.7 macrophages attachment assay shows that 1 μm- and 500 nm-pillars improved cell attachment than the planar surface. Macrophage fusion assay shows that the number of multinucleated cells on 1 μm- and 500 nm-pillars was approximately 1.4 to 1.5 times higher than that on the planar surface. However, 2 μm-pillar was ineffective in both the assays. Interestingly, the expression of F-actin and vinculin in mononuclear and multinucleated cells on the pillars appeared ring shaped along the circumference of pillars, indicating frustrated phagocytosis. We suggest that fine micro-patterning of the substrate could be one of the factors that control cell attachment and fusion of RAW 264.7 cells.; (主査) 教授 吉田 靖弘, 教授 網塚 憲生, 准教授 佐藤 嘉晃; 歯学研究科(口腔医学専攻)
• DOI: 10.14943/doctoral.k13477; https://doi.org/10.14943/doctoral.k13477
• Persistent ID (NDL): info:ndljp/pid/11479172; https://dl.ndl.go.jp/pid/11479172
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-04-06T03:04:11+09:00
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://dx.doi.org/10.14943/doctoral.k13477; http://hdl.handle.net/2115/77059
• Is Referenced By (URI): http://hdl.handle.net/2115/73973
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• Summary, etc.: Macrophage-dependent immune response to the implant surface sometimes results in failure of implant. Macrophage behaviors are sensitive to the topolog...