Cystic Fibrosis Transmembrane Conductance Regulator Reduces Microtubule-Dependent Campylobacter jejuni Invasion

• 資料種別: 博士論文
• ISSN: 1098-5522
• タイトル: Cystic Fibrosis Transmembrane Conductance Regulator Reduces Microtubule-Dependent Campylobacter jejuni Invasion
• 巻次・部編番号: 85 10
• 著者・編者: Kido, Junko; Shimohata, Takaaki; Amano, Sachie; Hatayama, Sho; Nguyen, Quoc Anh; Sato, Yuri; Kanda, Yuna; Tentaku, Aya; Fukushima, Shiho; Nakahashi, Mutsumi; Uebanso, Takashi; Mawatari, Kazuaki; Takahashi, Akira
• 著者標目: Kido, Junko; Shimohata, Takaaki; Amano, Sachie; Hatayama, Sho; Nguyen, Quoc Anh; Sato, Yuri; Kanda, Yuna; Tentaku, Aya; Fukushima, Shiho; Nakahashi, Mutsumi; Uebanso, Takashi; Mawatari, Kazuaki; Takahashi, Akira
• 出版事項: American Society for Microbiology
• 出版年月日等: 2017-09-20
• 出版年（W3CDTF）: 2017-09-20
• 並列タイトル等: Cystic Fibrosis Transmembrane Conductance Regulatorは微小管依存的なカンピロバクター・ジェジュニの侵入を抑制する; Role of CFTR in Campylobacter jejuni infection
• タイトル（掲載誌）: Infection and Immunity
• 授与機関名: 徳島大学
• 授与年月日: 2020-03-23
• 授与年月日（W3CDTF）: 2020-03-23
• 報告番号: 甲第3409号
• 学位: 博士（栄養学）
• 博論授与番号: 甲第3409号
• 本文の言語コード: eng
• 件名標目: CFTR; Campylobacter jejuni; cell invasion; host response to infection; intracellular bacteria; microtubule
• 対象利用者: 一般
• 一般注記: Campylobacter jejuni (C. jejuni) is gastroenteritis inducible food-born pathogen. Invasion and adhesion process are essential for leading gastroenteritis in C. jejuni infection process. As against bacterial strategy for efficacy invasion and adhesion, mucosal layer play a key role in defense systems, which modulated by several ion channels and transporters mediated water flux on the intestine. Cystic fibrosis transmembrane conductance regulator (CFTR) play the main role in waterfulux in intestine, and it closely related with bacterial clearance. We previously reported that C. jejuni infection suppresses CFTR channel activity in intestinal epithelial cells, however the mechanism and importance of this suppression is unclear. This study seeks to elucidate the role of CFTR in C. jejuni-infection. Using HEK293 cells that stably express wild type and mutated CFTR, we found that CFTR attenuated C. jejuni invasion, it was not involved bacterial adhesion or intracellular survival but associated with microtubule-dependent cellular transport. Moreover we revealed that CFTR attenuated function of microtubule motor protein but not microtubule stability, which causes inhibition of C. jejuni-invasion. Meanwhile, the CFTR mutant G551D-CFTR, which has defects in channel activity, suppressed C. jejuni-invasion, whereasΔF508-CFTR, which has defects in maturation, did not suppress, suggesting that CFTR suppression of C. jejuni-invasion is related to CFTR maturation but not channel activity.Taken together, mature CFTR inhibited C. jejuni invasion by regulating microtubule-mediated pathways. We suggest that CFTR plays a critical role in cellular defenses against C. jejuni-invasion, and CFTR suppression may be an initial step in promoting cellular invasion during C. jejuni-infection.
• DOI: info:doi/10.1128/IAI.00311-17; https://doi.org/info:doi/10.1128/IAI.00311-17
• 国立国会図書館永続的識別子: info:ndljp/pid/11498506; https://dl.ndl.go.jp/pid/11498506
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2020-06-07T00:39:19+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://repo.lib.tokushima-u.ac.jp/114676
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp