Cystic Fibrosis Transmembrane Conductance Regulator Reduces Microtubule-Dependent Campylobacter jejuni Invasion

• Material Type: 博士論文
• ISSN: 1098-5522
• Title: Cystic Fibrosis Transmembrane Conductance Regulator Reduces Microtubule-Dependent Campylobacter jejuni Invasion
• Volume: 85 10
• Author/Editor: Kido, Junko; Shimohata, Takaaki; Amano, Sachie; Hatayama, Sho; Nguyen, Quoc Anh; Sato, Yuri; Kanda, Yuna; Tentaku, Aya; Fukushima, Shiho; Nakahashi, Mutsumi; Uebanso, Takashi; Mawatari, Kazuaki; Takahashi, Akira
• Author Heading: Kido, Junko; Shimohata, Takaaki; Amano, Sachie; Hatayama, Sho; Nguyen, Quoc Anh; Sato, Yuri; Kanda, Yuna; Tentaku, Aya; Fukushima, Shiho; Nakahashi, Mutsumi; Uebanso, Takashi; Mawatari, Kazuaki; Takahashi, Akira
• Publication, Distribution, etc.: American Society for Microbiology
• Publication Date: 2017-09-20
• Publication Date (W3CDTF): 2017-09-20
• Alternative Title: Cystic Fibrosis Transmembrane Conductance Regulatorは微小管依存的なカンピロバクター・ジェジュニの侵入を抑制する; Role of CFTR in Campylobacter jejuni infection
• Periodical title: Infection and Immunity
• Degree grantor/type: 徳島大学
• Date Granted: 2020-03-23
• Date Granted (W3CDTF): 2020-03-23
• Dissertation Number: 甲第3409号
• Degree Type: 博士（栄養学）
• Conferring No. (Dissertation): 甲第3409号
• Text Language Code: eng
• Subject Heading: CFTR; Campylobacter jejuni; cell invasion; host response to infection; intracellular bacteria; microtubule
• Target Audience: 一般
• Note (General): Campylobacter jejuni (C. jejuni) is gastroenteritis inducible food-born pathogen. Invasion and adhesion process are essential for leading gastroenteritis in C. jejuni infection process. As against bacterial strategy for efficacy invasion and adhesion, mucosal layer play a key role in defense systems, which modulated by several ion channels and transporters mediated water flux on the intestine. Cystic fibrosis transmembrane conductance regulator (CFTR) play the main role in waterfulux in intestine, and it closely related with bacterial clearance. We previously reported that C. jejuni infection suppresses CFTR channel activity in intestinal epithelial cells, however the mechanism and importance of this suppression is unclear. This study seeks to elucidate the role of CFTR in C. jejuni-infection. Using HEK293 cells that stably express wild type and mutated CFTR, we found that CFTR attenuated C. jejuni invasion, it was not involved bacterial adhesion or intracellular survival but associated with microtubule-dependent cellular transport. Moreover we revealed that CFTR attenuated function of microtubule motor protein but not microtubule stability, which causes inhibition of C. jejuni-invasion. Meanwhile, the CFTR mutant G551D-CFTR, which has defects in channel activity, suppressed C. jejuni-invasion, whereasΔF508-CFTR, which has defects in maturation, did not suppress, suggesting that CFTR suppression of C. jejuni-invasion is related to CFTR maturation but not channel activity.Taken together, mature CFTR inhibited C. jejuni invasion by regulating microtubule-mediated pathways. We suggest that CFTR plays a critical role in cellular defenses against C. jejuni-invasion, and CFTR suppression may be an initial step in promoting cellular invasion during C. jejuni-infection.
• DOI: info:doi/10.1128/IAI.00311-17; https://doi.org/info:doi/10.1128/IAI.00311-17
• Persistent ID (NDL): info:ndljp/pid/11498506; https://dl.ndl.go.jp/pid/11498506
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-06-07T00:39:19+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/114676
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp