Exogenous Administration of Low-Dose Lipopolysaccharide Potentiates Liver Fibrosis in a Choline-Deficient l-Amino-Acid-Defined Diet-Induced Murine Steatohepatitis Model.

• 資料種別: 博士論文
• ISSN: 1422-0067
• タイトル: Exogenous Administration of Low-Dose Lipopolysaccharide Potentiates Liver Fibrosis in a Choline-Deficient l-Amino-Acid-Defined Diet-Induced Murine Steatohepatitis Model.
• 巻次・部編番号: 20 11
• 著者・編者: Nakanishi, Keisuke; Kaji, Kosuke; Kitade, Mitsuteru; Kubo, Takuya; Furukawa, Masanori; Saikawa, Soichiro; Shimozato, Naotaka; Sato, Shinya; Seki, Kenichiro; Kawaratani, Hideto; Moriya, Kei; Namisaki, Tadashi; Yoshiji, Hitoshi
• 著者標目: Nakanishi, Keisuke; Kaji, Kosuke; Kitade, Mitsuteru; Kubo, Takuya; Furukawa, Masanori; Saikawa, Soichiro; Shimozato, Naotaka; Sato, Shinya; Seki, Kenichiro; Kawaratani, Hideto; Moriya, Kei; Namisaki, Tadashi; Yoshiji, Hitoshi
• 出版事項: MDPI
• 出版年月日等: 2019-06-03; 20 11
• 出版年（W3CDTF）: 2019-06-03
• 並列タイトル等: リポポリサッカライドの外因性投与はコリン欠乏 L-アミノ酸置換食誘発脂肪性肝炎モデルマウスにおいて肝線維化を促進する
• タイトル（掲載誌）: International journal of molecular sciences
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2020-03-16
• 授与年月日（W3CDTF）: 2020-03-16
• 報告番号: 甲第738号
• 学位: 博士（医学）
• 博論授与番号: 甲第738号
• 本文の言語コード: eng
• 件名標目: nonalcoholic steatohepatitis; lipopolysaccharide; choline-deficient l-amino-acid-defined diet; toll-like receptor; NF-κB; fibrosis
• 対象利用者: 一般
• 一般注記: type:Thesis; Various rodent models have been proposed for basic research; however, the pathogenesis of human nonalcoholic steatohepatitis (NASH) is difficult to closely mimic. Lipopolysaccharide (LPS) has been reported to play a pivotal role in fibrosis development during NASH progression via activation of toll-like receptor 4 (TLR4) signaling. This study aimed to clarify the impact of low-dose LPS challenge on NASH pathological progression and to establish a novel murine NASH model. C57BL/6J mice were fed a choline-deficient l-amino-acid-defined (CDAA) diet to induce NASH, and low-dose LPS (0.5 mg/kg) was intraperitoneally injected thrice a week. CDAA-fed mice showed hepatic CD14 overexpression, and low-dose LPS challenge enhanced TLR4/NF-κB signaling activation in the liver of CDAA-fed mice. LPS challenge potentiated CDAA-diet-mediated insulin resistance, hepatic steatosis with upregulated lipogenic genes, and F4/80-positive macrophage infiltration with increased proinflammatory cytokines. It is noteworthy that LPS administration extensively boosted pericellular fibrosis with the activation of hepatic stellate cells in CDAA-fed mice. Exogenous LPS administration exacerbated pericellular fibrosis in CDAA-mediated steatohepatitis in mice. These findings suggest a key role for LPS/TLR4 signaling in NASH progression, and the authors therefore propose this as a suitable model to mimic human NASH.; 博士（医学）・甲第738号・令和2年3月16日; © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).; identifier:International journal of molecular sciences Vol.20 No.11 Article No.2724 (2019 Jun); identifier:14220067; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3732; identifier:International journal of molecular sciences, 20(11): Article No.2724
• DOI: info:doi/10.3390/ijms20112724; https://doi.org/info:doi/10.3390/ijms20112724
• 国立国会図書館永続的識別子: info:ndljp/pid/11570065; https://dl.ndl.go.jp/pid/11570065
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2020-11-10T19:58:22+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://hdl.handle.net/10564/3732
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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