Exogenous Administration of Low-Dose Lipopolysaccharide Potentiates Liver Fibrosis in a Choline-Deficient l-Amino-Acid-Defined Diet-Induced Murine Steatohepatitis Model.

• Material Type: 博士論文
• ISSN: 1422-0067
• Title: Exogenous Administration of Low-Dose Lipopolysaccharide Potentiates Liver Fibrosis in a Choline-Deficient l-Amino-Acid-Defined Diet-Induced Murine Steatohepatitis Model.
• Volume: 20 11
• Author/Editor: Nakanishi, Keisuke; Kaji, Kosuke; Kitade, Mitsuteru; Kubo, Takuya; Furukawa, Masanori; Saikawa, Soichiro; Shimozato, Naotaka; Sato, Shinya; Seki, Kenichiro; Kawaratani, Hideto; Moriya, Kei; Namisaki, Tadashi; Yoshiji, Hitoshi
• Author Heading: Nakanishi, Keisuke; Kaji, Kosuke; Kitade, Mitsuteru; Kubo, Takuya; Furukawa, Masanori; Saikawa, Soichiro; Shimozato, Naotaka; Sato, Shinya; Seki, Kenichiro; Kawaratani, Hideto; Moriya, Kei; Namisaki, Tadashi; Yoshiji, Hitoshi
• Publication, Distribution, etc.: MDPI
• Publication Date: 2019-06-03; 20 11
• Publication Date (W3CDTF): 2019-06-03
• Alternative Title: リポポリサッカライドの外因性投与はコリン欠乏 L-アミノ酸置換食誘発脂肪性肝炎モデルマウスにおいて肝線維化を促進する
• Periodical title: International journal of molecular sciences
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2020-03-16
• Date Granted (W3CDTF): 2020-03-16
• Dissertation Number: 甲第738号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第738号
• Text Language Code: eng
• Subject Heading: nonalcoholic steatohepatitis; lipopolysaccharide; choline-deficient l-amino-acid-defined diet; toll-like receptor; NF-κB; fibrosis
• Target Audience: 一般
• Note (General): type:Thesis; Various rodent models have been proposed for basic research; however, the pathogenesis of human nonalcoholic steatohepatitis (NASH) is difficult to closely mimic. Lipopolysaccharide (LPS) has been reported to play a pivotal role in fibrosis development during NASH progression via activation of toll-like receptor 4 (TLR4) signaling. This study aimed to clarify the impact of low-dose LPS challenge on NASH pathological progression and to establish a novel murine NASH model. C57BL/6J mice were fed a choline-deficient l-amino-acid-defined (CDAA) diet to induce NASH, and low-dose LPS (0.5 mg/kg) was intraperitoneally injected thrice a week. CDAA-fed mice showed hepatic CD14 overexpression, and low-dose LPS challenge enhanced TLR4/NF-κB signaling activation in the liver of CDAA-fed mice. LPS challenge potentiated CDAA-diet-mediated insulin resistance, hepatic steatosis with upregulated lipogenic genes, and F4/80-positive macrophage infiltration with increased proinflammatory cytokines. It is noteworthy that LPS administration extensively boosted pericellular fibrosis with the activation of hepatic stellate cells in CDAA-fed mice. Exogenous LPS administration exacerbated pericellular fibrosis in CDAA-mediated steatohepatitis in mice. These findings suggest a key role for LPS/TLR4 signaling in NASH progression, and the authors therefore propose this as a suitable model to mimic human NASH.; 博士（医学）・甲第738号・令和2年3月16日; © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).; identifier:International journal of molecular sciences Vol.20 No.11 Article No.2724 (2019 Jun); identifier:14220067; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3732; identifier:International journal of molecular sciences, 20(11): Article No.2724
• DOI: info:doi/10.3390/ijms20112724; https://doi.org/info:doi/10.3390/ijms20112724
• Persistent ID (NDL): info:ndljp/pid/11570065; https://dl.ndl.go.jp/pid/11570065
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-11-10T19:58:22+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10564/3732
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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