Ischemic postconditioning prevents surge of presynaptic glutamate release by activating mitochondrial ATP-dependent potassium channels in the mouse hippocampus.

• 資料種別: 博士論文
• ISSN: 1932-6203
• タイトル: Ischemic postconditioning prevents surge of presynaptic glutamate release by activating mitochondrial ATP-dependent potassium channels in the mouse hippocampus.
• 巻次・部編番号: 14 4
• 著者・編者: Yokoyama, Shohei; Nakagawa, Ichiro; Ogawa, Yoichi; Morisaki, Yudai; Motoyama, Yasushi; Park, Young Su; Saito, Yasuhiko; Nakase, Hiroyuki
• 著者標目: Yokoyama, Shohei; Nakagawa, Ichiro; Ogawa, Yoichi; Morisaki, Yudai; Motoyama, Yasushi; Park, Young Su; Saito, Yasuhiko; Nakase, Hiroyuki
• 出版事項: Public Library of Science
• 出版年月日等: 2019-04-12; 14 4
• 出版年（W3CDTF）: 2019-04-12
• 並列タイトル等: マウス海馬神経細胞におけるIschemic postconditioning はミトコンドリア局在KATP チャンネルを介してグルタミン酸放出を抑制し神経保護効果をもたらす。
• タイトル（掲載誌）: PloS one
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2020-12-24
• 授与年月日（W3CDTF）: 2020-12-24
• 報告番号: 乙第1484号
• 学位: 博士（医学）
• 博論授与番号: 乙第1484号
• 本文の言語コード: eng
• 対象利用者: 一般
• 一般注記: type:Thesis; A mild ischemic load applied after a lethal ischemic insult reduces the subsequent ischemia-reperfusion injury, and is called ischemic postconditioning (PostC). We studied the effect of ischemic PostC on synaptic glutamate release using a whole-cell patch-clamp technique. We recorded spontaneous excitatory post-synaptic currents (sEPSCs) from CA1 pyramidal cells in mouse hippocampal slices. The ischemic load was perfusion of artificial cerebrospinal fluid (ACSF) equilibrated with mixed gas (95% N2 and 5% CO2). The ischemic load was applied for 7.5 min, followed by ischemic PostC 30 s later, consisting of three cycles of 15 s of reperfusion and 15 s of re-ischemia. We found that a surging increase in sEPSCs frequency occurred during the immediate-early reperfusion period after the ischemic insult. We found a significant positive correlation between cumulative sEPSCs and the number of dead CA1 neurons (r = 0.70; p = 0.02). Ischemic PostC significantly suppressed this surge of sEPSCs. The mitochondrial KATP (mito-KATP) channel opener, diazoxide, also suppressed the surge of sEPSCs when applied for 15 min immediately after the ischemic load. The mito-KATP channel blocker, 5-hydroxydecanoate (5-HD), significantly attenuated the suppressive effect of both ischemic PostC and diazoxide application on the surge of sEPSCs. These results suggest that the opening of mito-KATP channels is involved in the suppressive effect of ischemic PostC on synaptic glutamate release and protection against neuronal death. We hypothesize that activation of mito-KATP channels prevents mitochondrial malfunction and breaks mutual facilitatory coupling between glutamate release and Ca2+ entry at presynaptic sites.; 博士（医学）・乙第1484号・令和2年12月24日; Copyright: © 2019 Yokoyama et al. This is an open access article distributed under the terms of the Creative Commons Attribution License(https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.; identifier:PloS one Vol.14 No.4 Article No.e0215104 (2019 Apr); identifier:19326203; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3829; identifier:PloS one, 14(4): Article No.e0215104
• DOI: info:doi/10.1371/journal.pone.0215104; https://doi.org/info:doi/10.1371/journal.pone.0215104
• 国立国会図書館永続的識別子: info:ndljp/pid/11674095; https://dl.ndl.go.jp/pid/11674095
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2021-05-23T18:24:43+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://hdl.handle.net/10564/3829
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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