Investigation of herbal extracts that have both OPH activity enhancing action and AGE crosslink cleaving activity

• 資料種別: 記事
• タイトル: Investigation of herbal extracts that have both OPH activity enhancing action and AGE crosslink cleaving activity
• 著者・編者: Hiroshige Kawai; Naoki Matsuo; Eiji Yuasa
• 出版事項: 日本 : 糖化ストレス研究会
• 出版年月日等: 2021-03-31
• 出版年（W3CDTF）: 2021-03-31
• 並列タイトル等: OPH活性増強とAGEs架橋切断の作用を併せ持つハーブエキスの探索
• タイトル（掲載誌）: Glycative stress research
• 巻号年月日等（掲載誌）: 8(1)
• 掲載巻: 8(1)
• ISSN（掲載誌）: 2188-3610
• ISSN-L（掲載誌）: 2188-3610
• 本文の言語コード: eng; jpn
• DOI: 10.24659/gsr.8.1_39; https://doi.org/10.24659/gsr.8.1_39
• 国立国会図書館永続的識別子: info:ndljp/pid/11703338; https://dl.ndl.go.jp/pid/11703338
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > その他; https://dl.ndl.go.jp/collections/B00035
• 収集根拠: オンライン資料収集制度
• 受理日（W3CDTF）: 2021-07-14T21:40:34+09:00
• 保存日（W3CDTF）: 2021-05-23
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: https://www.toukastress.jp/webj/
• 掲載誌（国立国会図書館永続的識別子）: info:ndljp/pid/11703333; https://dl.ndl.go.jp/pid/11703333
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• 要約等: Objective: Accumulation of advanced glycation end products (AGEs) produced by glycative stress promotes the progression of age-related diseases. The potential methods to reduce already accumulated AGEs include enhancing the oxidized protein hydrolase (OPH) that originally exists in the body to promote the decomposition of the AGE-derived protein, and directly acting on the AGE crosslinks to cleave them. Therefore, this study investigated herbs that work to enhance OPH activity as well as to cleave AGE crosslinks. Methods: Hot water (80°C) extracts of 6 kinds of herbs were used as the samples. 1) N-acetyl-L-alanine p-nitroanilide (AAPA) was used as a substrate for the OPH activity. The herbal extracts, OPH solution, AAPA solution and Tris-HCl buffer solution were mixed at a ratio of 1: 1: 2: 21 and left to react for 24 hours at 37°C. Then, the absorbance of p-nitroaniline, which is produced by enzymatic degradation, at 405 nm was measured. 2) 1-Phenyl-1,2-propanedione (PPD), which has an α-diketone structure as a substrate, was used as the AGE crosslink cleaving model. The herbal extracts, PPD solution, and phosphate buffer solution were mixed at a ratio of 5 : 1 : 4 and left to react at 37°C for 8 hours. Then, hydrochloric acid was added to the solution and the solution was centrifuged to obtain a supernatant. The benzoic acid in the supernatant, which was produced upon decomposition of PPD by the herbal extracts, was measured using the HPLC method. Results: The OPH activity enhancing action was found to be stronger in the order of fenugreek (Trigo nella foenum-graecum) seeds (106.9%), fennel (Foeniculum vulgare) seeds (81.8%), hibiscus (Hibiscus sabdariffa) calyxes and bracts (63.0%). The AGE crosslink cleaving action was stronger in the order of fennel seeds (39.0%), lemon balm (Melissa officinalis) leaves (29.6%), rosemary (Rosmarinus officinalis) leaves and stems (26.6%). In combination, OPH activity was on the attenuated side when even one herbal extract that attenuates the OPH activity was included. Conclusion: Fennel, fenugreek and hibiscus were found to have both OPH activity enhancing action and AGE crosslink cleaving activity. It was also suggested that depending on the combination of these herbs and their ratio, it may optimize both effects.
• DOI: 10.24659/gsr.8.1_39
• オンライン閲覧公開範囲: インターネット公開
• 連携機関・データベース: 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp

• 要約等: Objective: Accumulation of advanced glycation end products (AGEs) produced by glycative stress promotes the progression of age-related diseases. The potential methods to reduce already accumulated AGEs include enhancing the oxidized protein hydrolase (OPH) that originally exists in the body to promote the decomposition of the AGE-derived protein, and directly acting on the AGE crosslinks to cleave them. Therefore, this study investigated herbs that work to enhance OPH activity as well as to cleave AGE crosslinks. Methods: Hot water (80°C) extracts of 6 kinds of herbs were used as the samples. 1) N-acetyl-L-alanine p-nitroanilide (AAPA) was used as a substrate for the OPH activity. The herbal extracts, OPH solution, AAPA solution and Tris-HCl buffer solution were mixed at a ratio of 1: 1: 2: 21 and left to react for 24 hours at 37°C. Then, the absorbance of p-nitroaniline, which is produced by enzymatic degradation, at 405 nm was measured. 2) 1-Phenyl-1,2-propanedione (PPD), which has an α-diketone structure as a substrate, was used as the AGE crosslink cleaving model. The herbal extracts, PPD solution, and phosphate buffer solution were mixed at a ratio of 5 : 1 : 4 and left to react at 37°C for 8 hours. Then, hydrochloric acid was added to the solution and the solution was centrifuged to obtain a supernatant. The benzoic acid in the supernatant, which was produced upon decomposition of PPD by the herbal extracts, was measured using the HPLC method. Results: The OPH activity enhancing action was found to be stronger in the order of fenugreek (Trigo nella foenum-graecum) seeds (106.9%), fennel (Foeniculum vulgare) seeds (81.8%), hibiscus (Hibiscus sabdariffa) calyxes and bracts (63.0%). The AGE crosslink cleaving action was stronger in the order of fennel seeds (39.0%), lemon balm (Melissa officinalis) leaves (29.6%), rosemary (Rosmarinus officinalis) leaves and stems (26.6%). In combination, OPH activity was on the attenuated side when even one herbal extract that attenuates the OPH activity was included. Conclusion: Fennel, fenugreek and hibiscus were found to have both OPH activity enhancing action and AGE crosslink cleaving activity. It was also suggested that depending on the combination of these herbs and their ratio, it may optimize both effects.
• DOI: 10.24659/gsr.8.1_39; https://doi.org/10.24659/gsr.8.1_39
• 関連情報（URI）: http://id.ndl.go.jp/bib/032133651; https://ndlsearch.ndl.go.jp/books/R000000004-I032133651; http://id.ndl.go.jp/digimeta/11703338; https://dl.ndl.go.jp/pid/11703338; https://search.jamas.or.jp/link/ui/2022048922
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: Japan Link Center; https://japanlinkcenter.org/top; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; CiNii Articles
• 書誌ID（NDLBibID）: 032133651; http://id.ndl.go.jp/bib/032133651; 11703338; http://id.ndl.go.jp/bib/11703338
• NII論文ID: 130008046229

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