Investigation of herbal extracts that have both OPH activity enhancing action and AGE crosslink cleaving activity

• Material Type: 記事
• Title: Investigation of herbal extracts that have both OPH activity enhancing action and AGE crosslink cleaving activity
• Author/Editor: Hiroshige Kawai; Naoki Matsuo; Eiji Yuasa
• Publication, Distribution, etc.: 日本 : 糖化ストレス研究会
• Publication Date: 2021-03-31
• Publication Date (W3CDTF): 2021-03-31
• Alternative Title: OPH活性増強とAGEs架橋切断の作用を併せ持つハーブエキスの探索
• Periodical title: Glycative stress research
• No. or year of volume/issue: 8(1)
• Volume: 8(1)
• ISSN (Periodical Title): 2188-3610
• ISSN-L (Periodical Title): 2188-3610
• Text Language Code: eng; jpn
• DOI: 10.24659/gsr.8.1_39; https://doi.org/10.24659/gsr.8.1_39
• Persistent ID (NDL): info:ndljp/pid/11703338; https://dl.ndl.go.jp/pid/11703338
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > その他; https://dl.ndl.go.jp/collections/B00035
• Acquisition Basis: オンライン資料収集制度
• Date Accepted (W3CDTF): 2021-07-14T21:40:34+09:00
• Date Captured (W3CDTF): 2021-05-23
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): https://www.toukastress.jp/webj/
• Periodical Title (Persistent ID (NDL)): info:ndljp/pid/11703333; https://dl.ndl.go.jp/pid/11703333
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• Summary, etc.: Objective: Accumulation of advanced glycation end products (AGEs) produced by glycative stress promotes the progression of age-related diseases. The potential methods to reduce already accumulated AGEs include enhancing the oxidized protein hydrolase (OPH) that originally exists in the body to promote the decomposition of the AGE-derived protein, and directly acting on the AGE crosslinks to cleave them. Therefore, this study investigated herbs that work to enhance OPH activity as well as to cleave AGE crosslinks. Methods: Hot water (80°C) extracts of 6 kinds of herbs were used as the samples. 1) N-acetyl-L-alanine p-nitroanilide (AAPA) was used as a substrate for the OPH activity. The herbal extracts, OPH solution, AAPA solution and Tris-HCl buffer solution were mixed at a ratio of 1: 1: 2: 21 and left to react for 24 hours at 37°C. Then, the absorbance of p-nitroaniline, which is produced by enzymatic degradation, at 405 nm was measured. 2) 1-Phenyl-1,2-propanedione (PPD), which has an α-diketone structure as a substrate, was used as the AGE crosslink cleaving model. The herbal extracts, PPD solution, and phosphate buffer solution were mixed at a ratio of 5 : 1 : 4 and left to react at 37°C for 8 hours. Then, hydrochloric acid was added to the solution and the solution was centrifuged to obtain a supernatant. The benzoic acid in the supernatant, which was produced upon decomposition of PPD by the herbal extracts, was measured using the HPLC method. Results: The OPH activity enhancing action was found to be stronger in the order of fenugreek (Trigo nella foenum-graecum) seeds (106.9%), fennel (Foeniculum vulgare) seeds (81.8%), hibiscus (Hibiscus sabdariffa) calyxes and bracts (63.0%). The AGE crosslink cleaving action was stronger in the order of fennel seeds (39.0%), lemon balm (Melissa officinalis) leaves (29.6%), rosemary (Rosmarinus officinalis) leaves and stems (26.6%). In combination, OPH activity was on the attenuated side when even one herbal extract that attenuates the OPH activity was included. Conclusion: Fennel, fenugreek and hibiscus were found to have both OPH activity enhancing action and AGE crosslink cleaving activity. It was also suggested that depending on the combination of these herbs and their ratio, it may optimize both effects.
• DOI: 10.24659/gsr.8.1_39
• Access Restrictions: インターネット公開
• Data Provider (Database): 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp

• Summary, etc.: Objective: Accumulation of advanced glycation end products (AGEs) produced by glycative stress promotes the progression of age-related diseases. The potential methods to reduce already accumulated AGEs include enhancing the oxidized protein hydrolase (OPH) that originally exists in the body to promote the decomposition of the AGE-derived protein, and directly acting on the AGE crosslinks to cleave them. Therefore, this study investigated herbs that work to enhance OPH activity as well as to cleave AGE crosslinks. Methods: Hot water (80°C) extracts of 6 kinds of herbs were used as the samples. 1) N-acetyl-L-alanine p-nitroanilide (AAPA) was used as a substrate for the OPH activity. The herbal extracts, OPH solution, AAPA solution and Tris-HCl buffer solution were mixed at a ratio of 1: 1: 2: 21 and left to react for 24 hours at 37°C. Then, the absorbance of p-nitroaniline, which is produced by enzymatic degradation, at 405 nm was measured. 2) 1-Phenyl-1,2-propanedione (PPD), which has an α-diketone structure as a substrate, was used as the AGE crosslink cleaving model. The herbal extracts, PPD solution, and phosphate buffer solution were mixed at a ratio of 5 : 1 : 4 and left to react at 37°C for 8 hours. Then, hydrochloric acid was added to the solution and the solution was centrifuged to obtain a supernatant. The benzoic acid in the supernatant, which was produced upon decomposition of PPD by the herbal extracts, was measured using the HPLC method. Results: The OPH activity enhancing action was found to be stronger in the order of fenugreek (Trigo nella foenum-graecum) seeds (106.9%), fennel (Foeniculum vulgare) seeds (81.8%), hibiscus (Hibiscus sabdariffa) calyxes and bracts (63.0%). The AGE crosslink cleaving action was stronger in the order of fennel seeds (39.0%), lemon balm (Melissa officinalis) leaves (29.6%), rosemary (Rosmarinus officinalis) leaves and stems (26.6%). In combination, OPH activity was on the attenuated side when even one herbal extract that attenuates the OPH activity was included. Conclusion: Fennel, fenugreek and hibiscus were found to have both OPH activity enhancing action and AGE crosslink cleaving activity. It was also suggested that depending on the combination of these herbs and their ratio, it may optimize both effects.
• DOI: 10.24659/gsr.8.1_39; https://doi.org/10.24659/gsr.8.1_39
• Related Material (URI): http://id.ndl.go.jp/bib/032133651; https://ndlsearch.ndl.go.jp/books/R000000004-I032133651; http://id.ndl.go.jp/digimeta/11703338; https://dl.ndl.go.jp/pid/11703338; https://search.jamas.or.jp/link/ui/2022048922
• Data Provider (Database): 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• Original Data Provider (Database): Japan Link Center; https://japanlinkcenter.org/top; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; CiNii Articles
• Bibliographic ID (NDL): 032133651; http://id.ndl.go.jp/bib/032133651; 11703338; http://id.ndl.go.jp/bib/11703338
• NAID: 130008046229

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