一般注記This study was designed to explore potential messenger RNA (mRNA)-based biomarkers for detecting vascular senescence that can be applicable to the diagnosis of atherosclerosis-related diseases. The study was performed in two parts, an in silico search for candidate genes utilizing multiple public databases, and wet-experiments to validate the utility of candidate mRNAs as biomarkers. The relationship between the candidate mRNAs and the clinical parameters was evaluated using real-time qPCR, brachial ankle pulse wave velocity (baPWV), ankle-brachial index (ABI) and carotid ultrasound. Five candidate genes (CCNA1, HSD17B2, ADRB1, CMPK2 and RNF213) were selected based on the in silico experiments. Only CMPK2 and RNF213 were detectable in the saliva of both the young (20-30 year old) and senior (over 65 years old) groups. The salivary CMPK2 expression in the senior group was 3.8-fold greater than that in the young group (p = 0.0371). When appropriate expression levels of CMPK2 were used as the cut-off values for detecting arterial stiffness in the young group and plaque formation in the senior group, the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy were in the range of 66.7% - 100%. These results suggest that salivary CMPK2 may be a promising biomarker for chronological aging, arterial stiffness, and atherosclerosis.
2019年度
コレクション(個別)国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
受理日(W3CDTF)2022-02-06T04:33:19+09:00
連携機関・データベース国立国会図書館 : 国立国会図書館デジタルコレクション