Detection of biomarkers on aging and vascular senescence in saliva

Persistent ID (NDL): info:ndljp/pid/12071503

Material type: 博士論文

Author: 濵田, 吉宏

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Publication date: 2020-03-06

Material Format: Digital

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Name of awarding university/degree: 大阪歯科大学,博士（歯学）

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Note (General)：: This study was designed to explore potential messenger RNA (mRNA)-based biomarkers for detecting vascular senescence that can be applicable to the dia...

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Material Type: 博士論文
Title: Detection of biomarkers on aging and vascular senescence in saliva
Author/Editor: 濵田, 吉宏
Author Heading: 濵田, 吉宏
Publication Date: 2020-03-06
Publication Date (W3CDTF): 2020-03-06
Alternative Title: 加齢と血管老化に関する唾液中バイオマーカーの検出
Degree grantor/type: 大阪歯科大学
Date Granted: 2020-03-06
Date Granted (W3CDTF): 2020-03-06
Dissertation Number: 甲第874号
Degree Type: 博士（歯学）
Conferring No. (Dissertation): 甲第874号
Text Language Code: eng
Subject Heading: Saliva｜Aging｜Diagnosis｜Vascular senescence｜mRNA; Arteriosclerosis｜Atherosclerosis
Target Audience: 一般
Note (General): This study was designed to explore potential messenger RNA (mRNA)-based biomarkers for detecting vascular senescence that can be applicable to the diagnosis of atherosclerosis-related diseases. The study was performed in two parts, an in silico search for candidate genes utilizing multiple public databases, and wet-experiments to validate the utility of candidate mRNAs as biomarkers. The relationship between the candidate mRNAs and the clinical parameters was evaluated using real-time qPCR, brachial ankle pulse wave velocity (baPWV), ankle-brachial index (ABI) and carotid ultrasound. Five candidate genes (CCNA1, HSD17B2, ADRB1, CMPK2 and RNF213) were selected based on the in silico experiments. Only CMPK2 and RNF213 were detectable in the saliva of both the young (20-30 year old) and senior (over 65 years old) groups. The salivary CMPK2 expression in the senior group was 3.8-fold greater than that in the young group (p = 0.0371). When appropriate expression levels of CMPK2 were used as the cut-off values for detecting arterial stiffness in the young group and plaque formation in the senior group, the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy were in the range of 66.7% - 100%. These results suggest that salivary CMPK2 may be a promising biomarker for chronological aging, arterial stiffness, and atherosclerosis.
2019年度
Persistent ID (NDL): info:ndljp/pid/12071503
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Collection: 障害者向け資料
Collection (Materials For Handicapped People:1): テキストデータ
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
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Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2022-02-06T04:33:19+09:00
Date Created (W3CDTF): 2020-04-28
Format (IMT): application/pdf
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Availability of remote photoduplication service: 可
Periodical Title (URI): http://id.nii.ac.jp/1392/00000237/
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