Distinct effects on the secretion of MTRAP and AMA1 in Plasmodium yoelii following deletion of acylated pleckstrin homology domain-containing protein

• 資料種別: 博士論文
• ISSN: 1383-5769
• タイトル: Distinct effects on the secretion of MTRAP and AMA1 in Plasmodium yoelii following deletion of acylated pleckstrin homology domain-containing protein
• 巻次・部編番号: 86
• 著者・編者: Chaiyawong, Nattawat
• 著者標目: Chaiyawong, Nattawat
• 出版事項: Elsevier B.V.
• 出版年月日等: 2021-12-01
• 出版年（W3CDTF）: 2021-12-01
• 並列タイトル等: ネズミマラリア原虫Plasmodium yoeliiのacylated pleckstrin homology domain-containing protein を欠損させるとMTRAP とAMA1の分泌に異なった影響を与える
• タイトル（掲載誌）: Parasitology International
• 授与機関名: Nagasaki University (長崎大学)
• 授与年月日: 2021-12-01
• 授与年月日（W3CDTF）: 2021-12-01
• 報告番号: 甲医歯薬第1385号
• 学位: 博士(医学)
• 博論授与番号: 甲医歯薬第1385号
• 本文の言語コード: eng
• 件名標目: Erythrocyte invasion; Exocytosis; Malaria; Microneme; PH-domain containing protein
• 対象利用者: 一般
• 一般注記: Plasmodium, the causative agents of malaria, are obligate intracellular organisms. In humans, pathogenesis is caused by the blood stage parasite, which multiplies within erythrocytes, thus erythrocyte invasion is an essential developmental step. Merozoite form parasites released into the blood stream coordinately secrets a panel of proteins from the microneme secretory organelles for gliding motility, establishment of a tight junction with a target naive erythrocyte, and subsequent internalization. A protein identified in Toxoplasma gondii facilitates microneme fusion with the plasma membrane for exocytosis; namely, acylated pleckstrin homology domain-containing protein (APH). To obtain insight into the differential microneme discharge by malaria parasites, in this study we analyzed the consequences of APH deletion in the rodent malaria model, Plasmodium yoelii, using a DiCre-based inducible knockout method. We found that APH deletion resulted in a reduction in parasite asexual growth and erythrocyte invasion, with some parasites retaining the ability to invade and grow without APH. APH deletion impaired the secretion of microneme proteins, MTRAP and AMA1, and upon contact with erythrocytes the secretion of MTRAP, but not AMA1, was observed. APH-deleted merozoites were able to attach to and deform erythrocytes, consistent with the observed MTRAP secretion. Tight junctions were formed, but echinocytosis after merozoite internalization into erythrocytes was significantly reduced, consistent with the observed absence of AMA1 secretion. Together with our observation that APH largely colocalized with MTRAP, but less with AMA1, we propose that APH is directly involved in MTRAP secretion; whereas any role of APH in AMA1 secretion is indirect in Plasmodium.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1385号 学位授与年月日:令和3年12月1日; Author: Nattawat Chaiyawong, Takahiro Ishizaki, Hassan Hakimi, Masahito Asada, Kazuhide Yahata, Osamu Kaneko; Citation: Parasitology International, 86, 102479; 2022; identifier:Nagasaki University (長崎大学), 博士(医学) (2021-12-01); http://hdl.handle.net/10069/00041146
• DOI: info:doi/10.1016/j.parint.2021.102479; https://doi.org/info:doi/10.1016/j.parint.2021.102479
• 国立国会図書館永続的識別子: info:ndljp/pid/12154142; https://dl.ndl.go.jp/pid/12154142
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2022-03-06T04:54:00+09:00
• 作成日（W3CDTF）: 2022-02-25
• 記録形式（IMT）: PDF
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 関連情報（URI）: http://hdl.handle.net/10069/00041146
• 掲載誌（URI）: http://hdl.handle.net/10069/00041272
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp