Distinct effects on the secretion of MTRAP and AMA1 in Plasmodium yoelii following deletion of acylated pleckstrin homology domain-containing protein

• Material Type: 博士論文
• ISSN: 1383-5769
• Title: Distinct effects on the secretion of MTRAP and AMA1 in Plasmodium yoelii following deletion of acylated pleckstrin homology domain-containing protein
• Volume: 86
• Author/Editor: Chaiyawong, Nattawat
• Author Heading: Chaiyawong, Nattawat
• Publication, Distribution, etc.: Elsevier B.V.
• Publication Date: 2021-12-01
• Publication Date (W3CDTF): 2021-12-01
• Alternative Title: ネズミマラリア原虫Plasmodium yoeliiのacylated pleckstrin homology domain-containing protein を欠損させるとMTRAP とAMA1の分泌に異なった影響を与える
• Periodical title: Parasitology International
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2021-12-01
• Date Granted (W3CDTF): 2021-12-01
• Dissertation Number: 甲医歯薬第1385号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医歯薬第1385号
• Text Language Code: eng
• Subject Heading: Erythrocyte invasion; Exocytosis; Malaria; Microneme; PH-domain containing protein
• Target Audience: 一般
• Note (General): Plasmodium, the causative agents of malaria, are obligate intracellular organisms. In humans, pathogenesis is caused by the blood stage parasite, which multiplies within erythrocytes, thus erythrocyte invasion is an essential developmental step. Merozoite form parasites released into the blood stream coordinately secrets a panel of proteins from the microneme secretory organelles for gliding motility, establishment of a tight junction with a target naive erythrocyte, and subsequent internalization. A protein identified in Toxoplasma gondii facilitates microneme fusion with the plasma membrane for exocytosis; namely, acylated pleckstrin homology domain-containing protein (APH). To obtain insight into the differential microneme discharge by malaria parasites, in this study we analyzed the consequences of APH deletion in the rodent malaria model, Plasmodium yoelii, using a DiCre-based inducible knockout method. We found that APH deletion resulted in a reduction in parasite asexual growth and erythrocyte invasion, with some parasites retaining the ability to invade and grow without APH. APH deletion impaired the secretion of microneme proteins, MTRAP and AMA1, and upon contact with erythrocytes the secretion of MTRAP, but not AMA1, was observed. APH-deleted merozoites were able to attach to and deform erythrocytes, consistent with the observed MTRAP secretion. Tight junctions were formed, but echinocytosis after merozoite internalization into erythrocytes was significantly reduced, consistent with the observed absence of AMA1 secretion. Together with our observation that APH largely colocalized with MTRAP, but less with AMA1, we propose that APH is directly involved in MTRAP secretion; whereas any role of APH in AMA1 secretion is indirect in Plasmodium.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1385号 学位授与年月日:令和3年12月1日; Author: Nattawat Chaiyawong, Takahiro Ishizaki, Hassan Hakimi, Masahito Asada, Kazuhide Yahata, Osamu Kaneko; Citation: Parasitology International, 86, 102479; 2022; identifier:Nagasaki University (長崎大学), 博士(医学) (2021-12-01); http://hdl.handle.net/10069/00041146
• DOI: info:doi/10.1016/j.parint.2021.102479; https://doi.org/info:doi/10.1016/j.parint.2021.102479
• Persistent ID (NDL): info:ndljp/pid/12154142; https://dl.ndl.go.jp/pid/12154142
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-03-06T04:54:00+09:00
• Date Created (W3CDTF): 2022-02-25
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Related Material (URI): http://hdl.handle.net/10069/00041146
• Periodical Title (URI): http://hdl.handle.net/10069/00041272
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp