Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells

• 資料種別: 博士論文
• ISSN: 1999-4923
• タイトル: Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells
• 巻次・部編番号: 13 8
• 著者・編者: 石井, 光寿
• 著者標目: 石井, 光寿
• 出版事項: MDPI
• 出版年月日等: 2021-12-01
• 出版年（W3CDTF）: 2021-12-01
• 並列タイトル等: 脂肪由来間葉系幹細胞による肺上皮細胞間バリア機能増強効果
• タイトル（掲載誌）: Pharmaceutics
• 授与機関名: Nagasaki University (長崎大学)
• 授与年月日: 2021-12-01
• 授与年月日（W3CDTF）: 2021-12-01
• 報告番号: 甲医歯薬第1376号
• 学位: 博士(医学)
• 博論授与番号: 甲医歯薬第1376号
• 本文の言語コード: eng
• 件名標目: mesenchymal stem/stromal cell; alveolar epithelial cell; barrier function; air-blood barrier; transepithelial electrical resistance; acute respiratory distress syndrome
• 対象利用者: 一般
• 一般注記: With the emergence of coronavirus disease-2019, researchers have gained interest in the therapeutic efficacy of mesenchymal stem/stromal cells (MSCs) in acute respiratory distress syndrome; however, the mechanisms of the therapeutic effects of MSCs are unclear. We have previously reported that adipose-derived MSCs (AD-MSCs) strengthen the barrier function of the pulmonary vessels in scaffold-based bioengineered rat lungs. In this study, we evaluated whether AD-MSCs could enhance the intercellular barrier function of lung epithelial cells in vitro using a transwell coculture system. Transepithelial electrical resistance (TEER) measurements revealed that the peak TEER value was significantly higher in the AD-MSC coculture group than in the AD-MSC non-coculture group. Similarly, the permeability coefficient was significantly decreased in the AD-MSC coculture group compared to that in the AD-MSC non-coculture group. Immunostaining of insert membranes showed that zonula occuldens-1 expression was significantly high at cell junctions in the AD-MSC coculture group. Moreover, cell junction-related gene profiling showed that the expression of some claudin genes, including claudin-4, was upregulated in the AD-MSC coculture group. Taken together, these results showed that AD-MSCs enhanced the barrier function between lung epithelial cells, suggesting that both direct adhesion and indirect paracrine effects strengthened the barrier function of lung alveolar epithelium in vitro.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1376号 学位授与年月日:令和3年12月1日; Author: Mitsutoshi Ishii, Tomoshi Tsuchiya, Ryoichiro Doi, Yoichi Morofuji, Takashi Fujimoto, Hideki Muto, Takashi Suematsu, Ryoichi Mori, Keitaro Matsumoto, Takuro Miyazaki, Koichi Tomoshige, Hironosuke Watanabe, Mayumi Iwatake and Takeshi Nagayasu; Citation: Pharmaceutics, 13(8), 1264; 2021; identifier:Nagasaki University (長崎大学), 博士(医学) (2021-12-01); http://hdl.handle.net/10069/00041137
• DOI: info:doi/10.3390/pharmaceutics13081264; https://doi.org/info:doi/10.3390/pharmaceutics13081264
• 国立国会図書館永続的識別子: info:ndljp/pid/12154278; https://dl.ndl.go.jp/pid/12154278
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2022-03-06T04:54:00+09:00
• 作成日（W3CDTF）: 2022-02-14
• 記録形式（IMT）: PDF
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 関連情報（URI）: http://hdl.handle.net/10069/00041137
• 掲載誌（URI）: http://hdl.handle.net/10069/00041263
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp