Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells

• Material Type: 博士論文
• ISSN: 1999-4923
• Title: Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells
• Volume: 13 8
• Author/Editor: 石井, 光寿
• Author Heading: 石井, 光寿
• Publication, Distribution, etc.: MDPI
• Publication Date: 2021-12-01
• Publication Date (W3CDTF): 2021-12-01
• Alternative Title: 脂肪由来間葉系幹細胞による肺上皮細胞間バリア機能増強効果
• Periodical title: Pharmaceutics
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2021-12-01
• Date Granted (W3CDTF): 2021-12-01
• Dissertation Number: 甲医歯薬第1376号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医歯薬第1376号
• Text Language Code: eng
• Subject Heading: mesenchymal stem/stromal cell; alveolar epithelial cell; barrier function; air-blood barrier; transepithelial electrical resistance; acute respiratory distress syndrome
• Target Audience: 一般
• Note (General): With the emergence of coronavirus disease-2019, researchers have gained interest in the therapeutic efficacy of mesenchymal stem/stromal cells (MSCs) in acute respiratory distress syndrome; however, the mechanisms of the therapeutic effects of MSCs are unclear. We have previously reported that adipose-derived MSCs (AD-MSCs) strengthen the barrier function of the pulmonary vessels in scaffold-based bioengineered rat lungs. In this study, we evaluated whether AD-MSCs could enhance the intercellular barrier function of lung epithelial cells in vitro using a transwell coculture system. Transepithelial electrical resistance (TEER) measurements revealed that the peak TEER value was significantly higher in the AD-MSC coculture group than in the AD-MSC non-coculture group. Similarly, the permeability coefficient was significantly decreased in the AD-MSC coculture group compared to that in the AD-MSC non-coculture group. Immunostaining of insert membranes showed that zonula occuldens-1 expression was significantly high at cell junctions in the AD-MSC coculture group. Moreover, cell junction-related gene profiling showed that the expression of some claudin genes, including claudin-4, was upregulated in the AD-MSC coculture group. Taken together, these results showed that AD-MSCs enhanced the barrier function between lung epithelial cells, suggesting that both direct adhesion and indirect paracrine effects strengthened the barrier function of lung alveolar epithelium in vitro.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1376号 学位授与年月日:令和3年12月1日; Author: Mitsutoshi Ishii, Tomoshi Tsuchiya, Ryoichiro Doi, Yoichi Morofuji, Takashi Fujimoto, Hideki Muto, Takashi Suematsu, Ryoichi Mori, Keitaro Matsumoto, Takuro Miyazaki, Koichi Tomoshige, Hironosuke Watanabe, Mayumi Iwatake and Takeshi Nagayasu; Citation: Pharmaceutics, 13(8), 1264; 2021; identifier:Nagasaki University (長崎大学), 博士(医学) (2021-12-01); http://hdl.handle.net/10069/00041137
• DOI: info:doi/10.3390/pharmaceutics13081264; https://doi.org/info:doi/10.3390/pharmaceutics13081264
• Persistent ID (NDL): info:ndljp/pid/12154278; https://dl.ndl.go.jp/pid/12154278
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-03-06T04:54:00+09:00
• Date Created (W3CDTF): 2022-02-14
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Related Material (URI): http://hdl.handle.net/10069/00041137
• Periodical Title (URI): http://hdl.handle.net/10069/00041263
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp