Noninvasive measurement of cell/colony motion using image analysis methods to evaluate the proliferative capacity of oral keratinocytes as a tool for quality control in regenerative medicine

• 資料種別: 博士論文
• タイトル: Noninvasive measurement of cell/colony motion using image analysis methods to evaluate the proliferative capacity of oral keratinocytes as a tool for quality control in regenerative medicine
• 著者・編者: Hoshikawa, Emi
• 著者標目: Hoshikawa, Emi
• 出版年月日等: 2021-03-23
• 出版年（W3CDTF）: 2021-03-23
• 並列タイトル等: 口腔ケラチノサイトの増殖能を評価するための画像解析法を用いた細胞/コロニー運動の非侵襲的測定 : 再生医療用細胞品質管理ツールとして
• 授与機関名: 新潟大学
• 授与年月日: 2021-03-23
• 授与年月日（W3CDTF）: 2021-03-23
• 報告番号: 甲第4874号
• 学位: 博士（歯学）
• 博論授与番号: 甲第4874号
• 本文の言語コード: eng
• 著者別名: 干川, 絵美
• 件名標目: Oral keratinocyte; quality control; regenerative medicine; cell/colony motion; image analysis
• 対象利用者: 一般
• 一般注記: Image-based cell/colony analyses offer promising solutions to compensate for the lack of quality control (QC) tools for noninvasive monitoring of cultured cells, a regulatory challenge in regenerative medicine. Here, the feasibility of two image analysis algorithms, optical flow and normalised cross-correlation, to noninvasively measure cell/colony motion in human primary oral keratinocytes for screening the proliferative capacity of cells in the early phases of cell culture were examined. We applied our software to movies converted from 96 consecutive time-lapse phase-contrast images of an oral keratinocyte culture. After segmenting the growing colonies, two indices were calculated based on each algorithm. The correlation between each index of the colonies and their proliferative capacity was evaluated. The software was able to assess cell/colony motion noninvasively, and each index reflected the observed cell kinetics. A positive linear correlation was found between cell/colony motion and proliferative capacity, indicating that both algorithms are potential tools for QC.; Journal of Tissue Engineering, 2019, 10, 1-12.; 新大院博(歯)第482号
• 国立国会図書館永続的識別子: info:ndljp/pid/12263510; https://dl.ndl.go.jp/pid/12263510
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2022-05-09T11:57:37+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://hdl.handle.net/10191/0002000370; https://niigata-u.repo.nii.ac.jp/records/2000370
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp