Noninvasive measurement of cell/colony motion using image analysis methods to evaluate the proliferative capacity of oral keratinocytes as a tool for quality control in regenerative medicine

Persistent ID (NDL): info:ndljp/pid/12263510

Material type: 博士論文

Author: Hoshikawa, Emi

Publisher: -

Publication date: 2021-03-23

Material Format: Digital

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Name of awarding university/degree: 新潟大学,博士（歯学）

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Note (General)：: Image-based cell/colony analyses offer promising solutions to compensate for the lack of quality control (QC) tools for noninvasive monitoring of cult...

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Material Type: 博士論文
Title: Noninvasive measurement of cell/colony motion using image analysis methods to evaluate the proliferative capacity of oral keratinocytes as a tool for quality control in regenerative medicine
Author/Editor: Hoshikawa, Emi
Author Heading: Hoshikawa, Emi
Publication Date: 2021-03-23
Publication Date (W3CDTF): 2021-03-23
Alternative Title: 口腔ケラチノサイトの増殖能を評価するための画像解析法を用いた細胞/コロニー運動の非侵襲的測定 : 再生医療用細胞品質管理ツールとして
Degree grantor/type: 新潟大学
Date Granted: 2021-03-23
Date Granted (W3CDTF): 2021-03-23
Dissertation Number: 甲第4874号
Degree Type: 博士（歯学）
Conferring No. (Dissertation): 甲第4874号
Text Language Code: eng
Alias of Author: 干川, 絵美
Subject Heading: Oral keratinocyte
quality control
regenerative medicine
cell/colony motion
image analysis
Target Audience: 一般
Note (General): Image-based cell/colony analyses offer promising solutions to compensate for the lack of quality control (QC) tools for noninvasive monitoring of cultured cells, a regulatory challenge in regenerative medicine. Here, the feasibility of two image analysis algorithms, optical flow and normalised cross-correlation, to noninvasively measure cell/colony motion in human primary oral keratinocytes for screening the proliferative capacity of cells in the early phases of cell culture were examined. We applied our software to movies converted from 96 consecutive time-lapse phase-contrast images of an oral keratinocyte culture. After segmenting the growing colonies, two indices were calculated based on each algorithm. The correlation between each index of the colonies and their proliferative capacity was evaluated. The software was able to assess cell/colony motion noninvasively, and each index reflected the observed cell kinetics. A positive linear correlation was found between cell/colony motion and proliferative capacity, indicating that both algorithms are potential tools for QC.
Journal of Tissue Engineering, 2019, 10, 1-12.
新大院博(歯)第482号
Persistent ID (NDL): info:ndljp/pid/12263510
https://dl.ndl.go.jp/pid/12263510
Collection: 障害者向け資料
Collection (Materials For Handicapped People:1): テキストデータ
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2022-05-09T11:57:37+09:00
Format (IMT): application/pdf
Access Restrictions: 国立国会図書館内限定公開
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Availability of remote photoduplication service: 可
Periodical Title (URI): http://hdl.handle.net/10191/0002000370
https://niigata-u.repo.nii.ac.jp/records/2000370
Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション
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