並列タイトル等発現Nタンパク質を用いた新型コロナウイルス特異的IgG抗体測定法の開発と評価
タイトル（掲載誌）International Journal of Environmental Research and Public Health
授与機関名Nagasaki University (長崎大学)
一般注記Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). Real-time RT-PCR is the most commonly used method for COVID-19 diagnosis. However, serological assays are urgently needed as complementary tools to RT-PCR. Hachim et al. 2020 and Burbelo et al. 2020 emonstrated that anti-nucleocapsid(N) SARS-CoV-2 antibodies are higher and appear earlier than the spike antibodies. Additionally, cross-reactive antibodies against N protein are more prevalent than those against spike protein. We developed a less cross-reactive immunoglobulin G (IgG) indirect ELISA by using a truncated recombinant SARS-CoV-2 N protein as assay antigen. A highly conserved region of coronaviruses N protein was deleted and the protein was prepared using an E. coli protein expression system. A total of 177 samples collected from COVID-19 suspected cases and 155 negative control sera collected during the pre-COVID-19 period were applied to evaluate the assay’s performance, with the plaque reduction neutralization test and the commercial SARS-CoV-2 spike protein IgG ELISA as gold standards. The SARS-CoV-2 N truncated protein-based ELISA showed similar sensitivity (91.1% vs. 91.9%) and specificity (93.8% vs. 93.8%) between the PRNT and spike IgG ELISA, as well as also higher specificity compared to the full-length N protein (93.8% vs. 89.9%). Our ELISA can be used for the diagnosis and surveillance of COVID-19.
長崎大学学位論文 学位記番号:博(医歯薬)甲第1438号 学位授与年月日:令和4年3月18日
Author: Pierre Nsele Mutantu, Mya Myat Ngwe Tun, Takeshi Nabeshima, Fuxun Yu, Patrick Kakoni Mukadi, Takeshi Tanaka, Masato Tashiro, Ayumi Fujita, Nobuhiro Kanie, Ryosaku Oshiro, Takahiro Takazono, Yoshifumi Imamura, Tatsuro Hirayama, Meng Ling Moi , Shingo Inoue, Koichi Izumikawa, Jiro Yasuda and Kouichi Morita
International Journal of Environmental Research and Public Health, 18(18), art.no. 9630; 2021
identifier:Nagasaki University (長崎大学), 博士(医学) (2022-03-18)
DOIinfo:doi/10.3390/ijerph18189630
コレクション（個別）国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
受理日（W3CDTF）2022-06-05T18:01:14+09:00
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