Development and Evaluation of Quantitative Immunoglobulin G Enzyme-Linked Immunosorbent Assay for the Diagnosis of Coronavirus Disease 2019 Using Truncated Recombinant Nucleocapsid Protein as Assay Antigen

• Material Type: 博士論文
• ISSN: 1660-4601
• Title: Development and Evaluation of Quantitative Immunoglobulin G Enzyme-Linked Immunosorbent Assay for the Diagnosis of Coronavirus Disease 2019 Using Truncated Recombinant Nucleocapsid Protein as Assay Antigen
• Volume: 18 18
• Author/Editor: Mutantu, Nsele Pierre
• Author Heading: Mutantu, Nsele Pierre
• Publication, Distribution, etc.: MDPI
• Publication Date: 2022-03-18; 18 18
• Publication Date (W3CDTF): 2022-03-18
• Alternative Title: 発現Nタンパク質を用いた新型コロナウイルス特異的IgG抗体測定法の開発と評価
• Periodical title: International Journal of Environmental Research and Public Health
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2022-03-18
• Date Granted (W3CDTF): 2022-03-18
• Dissertation Number: 甲医歯薬第1438号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医歯薬第1438号
• Text Language Code: eng
• Subject Heading: SARS-CoV-2; nucleocapsid protein; IgG indirect ELISA; plaque reduction neutralization test
• Target Audience: 一般
• Note (General): Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). Real-time RT-PCR is the most commonly used method for COVID-19 diagnosis. However, serological assays are urgently needed as complementary tools to RT-PCR. Hachim et al. 2020 and Burbelo et al. 2020 emonstrated that anti-nucleocapsid(N) SARS-CoV-2 antibodies are higher and appear earlier than the spike antibodies. Additionally, cross-reactive antibodies against N protein are more prevalent than those against spike protein. We developed a less cross-reactive immunoglobulin G (IgG) indirect ELISA by using a truncated recombinant SARS-CoV-2 N protein as assay antigen. A highly conserved region of coronaviruses N protein was deleted and the protein was prepared using an E. coli protein expression system. A total of 177 samples collected from COVID-19 suspected cases and 155 negative control sera collected during the pre-COVID-19 period were applied to evaluate the assay’s performance, with the plaque reduction neutralization test and the commercial SARS-CoV-2 spike protein IgG ELISA as gold standards. The SARS-CoV-2 N truncated protein-based ELISA showed similar sensitivity (91.1% vs. 91.9%) and specificity (93.8% vs. 93.8%) between the PRNT and spike IgG ELISA, as well as also higher specificity compared to the full-length N protein (93.8% vs. 89.9%). Our ELISA can be used for the diagnosis and surveillance of COVID-19.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1438号 学位授与年月日:令和4年3月18日; Author: Pierre Nsele Mutantu, Mya Myat Ngwe Tun, Takeshi Nabeshima, Fuxun Yu, Patrick Kakoni Mukadi, Takeshi Tanaka, Masato Tashiro, Ayumi Fujita, Nobuhiro Kanie, Ryosaku Oshiro, Takahiro Takazono, Yoshifumi Imamura, Tatsuro Hirayama, Meng Ling Moi , Shingo Inoue, Koichi Izumikawa, Jiro Yasuda and Kouichi Morita; International Journal of Environmental Research and Public Health, 18(18), art.no. 9630; 2021; identifier:Nagasaki University (長崎大学), 博士(医学) (2022-03-18)
• DOI: info:doi/10.3390/ijerph18189630; https://doi.org/info:doi/10.3390/ijerph18189630
• Persistent ID (NDL): info:ndljp/pid/12296681; https://dl.ndl.go.jp/pid/12296681
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-06-05T18:01:14+09:00
• Date Created (W3CDTF): 2022-05-26
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Related Material (URI): http://hdl.handle.net/10069/00041441
• Periodical Title (URI): http://hdl.handle.net/10069/00041556
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp