Effect of Non-Thermal Atmospheric Pressure Plasma on Differentiation Potential of Human Deciduous Dental Pulp Fibroblast-like Cells

• 資料種別: 博士論文
• タイトル: Effect of Non-Thermal Atmospheric Pressure Plasma on Differentiation Potential of Human Deciduous Dental Pulp Fibroblast-like Cells
• 著者・編者: 奥野, 真江
• 著者標目: 奥野, 真江
• 出版年月日等: 2022-03-04
• 出版年（W3CDTF）: 2022-03-04
• 並列タイトル等: 非熱的大気圧プラズマがヒト乳歯歯髄線維芽細胞の分化能に及ぼす影響
• 授与機関名: 大阪歯科大学
• 授与年月日: 2022-03-04
• 授与年月日（W3CDTF）: 2022-03-04
• 報告番号: 甲第920号
• 学位: 博士（歯学）
• 博論授与番号: 甲第920号
• 本文の言語コード: eng
• 件名標目: non-thermal atmospheric pressure plasma (NTAPP)｜human deciduous dental pulp fibroblast-like cells｜regenerative medicine
• 対象利用者: 一般
• 一般注記: Human mesenchymal stem cells can differentiate into various cell types and are useful for applications in regenerative medicine. Previous studies indicated that dental pulp exfoliated from deciduous teeth is a valuable alternative for dental tissue engineering because it contains stem cells with a relatively high proliferation rate. For clinical application, it is necessary to rapidly obtain a sufficient number of cells in vitro and maintain their undifferentiated state; however, the abundance of stem cells in the dental pulp tissue is limited. Non-thermal atmospheric pressure plasma (NTAPP) has been applied in regenerative medicine because it activates cell proliferation. Here, we examined the effects of NTAPP to activate the proliferation of human deciduous dental pulp fibroblast-like cells (hDDPFs) in vitro. Compared with untreated cells, NTAPP increased cell proliferation by 1.3-fold, significantly upregulated well-known pluripotent genes for stemness (e.g., Oct4, Sox2, and Nanog), and activated the expression of stem cell-specific surface markers (e.g., CD105). Overall, NTAPP activated the proliferation of various mesodermal-derived human adult stem cells while maintaining their pluripotency and stemness. In conclusion, NTAPP is a potential tool to expand the population of various adult stem cells in vitro for medical applications.; 2021年度; 機関リポジトリ記載の権利情報: CC BY-NC-ND
• 国立国会図書館永続的識別子: info:ndljp/pid/12296876; https://dl.ndl.go.jp/pid/12296876
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2022-06-05T18:01:14+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://id.nii.ac.jp/1392/00000297/
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp