並列タイトル等卵巣癌関連線維芽細胞と対側の正常卵巣由来間葉系幹細胞における生物学的特性の相違に関する検討
授与機関名Nagasaki University (長崎大学)
一般注記Background/Aim: The aim of this study was to clarify the biological differences between ovarian cancerassociated fibroblasts (OCa-CAFs) and normal ovary-derived mesenchymal stem cells (NO-MSCs). Materials and Methods: Surgically resected ovarian cancer and contralateral normal ovarian tissue samples were cut into small pieces for culture as “explants”. The number of outgrown cells, their proliferative kinetics, and expression levels of cell surface markers of CAFs, as well as three miRNAs in OCa-CAFs and NO-MSCs were compared directly. Differentially expressed genes between both groups were also investigated. Results: Comparable numbers of outgrown cells were harvested from both groups. Significantly higher expression of α-smooth muscle actin and miR-142 was found in OCa-CAFs, which decreased significantly during ex vivo cell expansion. A total of 21 differentially expressed genes were identified between both groups. Conclusion: OCa-CAFs showed different biological properties in direct comparison with NO-MSCs, which might play major roles in the pathogenesis of ovarian cancer.
長崎大学学位論文 学位記番号:博(医歯薬)甲第1454号 学位授与年月日:令和4年6月1日
Author: Kazuaki Ohashi, Tao-Sheng Li, Shoko Miura, Yuri Hasegawa and Kiyonori Miura
Citation: ANTICANCER RESEARCH, 42(4), pp.1729-1737; 2022
identifier:Nagasaki University (長崎大学), 博士(医学) (2022-06-01)
http://hdl.handle.net/10069/00041584
DOIinfo:doi/10.21873/anticanres.15649
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